Cervical cancer is the third most common gynecologic cancer in the world. Exploration of the molecular mechanism underlying cervical cancer pathogenesis will provide new insights into the development of novel therapies. In this study, we were aimed to characterize a novel miRNA in cervical cancer tumorigenesis. First, we measured the expressional change of miR-144-3p in clinical tissues and cancer cells. Second, we employed cell proliferation, cell migration, and invasion assays to understand its functional role in cervical cancer. Then, we confirmed in vitro findings in xenograft cancer model. Last, we mapped out a downstream target of miR-144-3p and validated its functional role in cancer cells. In the results, miR-144-3p was found significantly downregulated in cervical cancer cells and tissues. Over-expressing miR-144-3p suppressed cancer cells growth and metastasis. Consistent with in vitro results, over-expressing miR-144-3p led to tumor growth inhibition in vivo. Further on, MAPK6 was identified as an endogenous target of miR-144-3p in cervical cancer. Knocking down MAPK6 inhibited cervical cancer cells proliferation, migration, and invasion potential. Our investigation was the first time to report miR-144-3p as a tumor suppressive miRNA in cervical cancer. It inhibited tumor growth by targeting MAKP6. The newly identified signalling axis may serve as novel therapeutic targets to manage cervical cancer.

中文翻译：

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MiR-144-3p：一种针对MAPK6的新型肿瘤抑制因子，用于宫颈癌。

宫颈癌是世界上第三大最常见的妇科癌症。对子宫颈癌发病机理的分子机制的探索将为新型疗法的发展提供新的见解。在这项研究中，我们旨在表征宫颈癌肿瘤发生中的新型miRNA。首先，我们测量了miR-144-3p在临床组织和癌细胞中的表达变化。其次，我们采用细胞增殖，细胞迁移和侵袭试验来了解其在宫颈癌中的功能。然后，我们证实了异种移植癌模型的体外发现。最后，我们绘制了miR-144-3p的下游靶标，并验证了其在癌细胞中的功能。结果表明，miR-144-3p在宫颈癌细胞和组织中显着下调。过表达的miR-144-3p抑制了癌细胞的生长和转移。与体外结果一致，miR-144-3p的过表达导致体内肿瘤生长受到抑制。进一步，MAPK6被确定为宫颈癌miR-144-3p的内源性靶标。敲除MAPK6可抑制子​​宫颈癌细胞的增殖，迁移和侵袭能力。我们的研究是首次将miR-144-3p报告为宫颈癌的抑癌miRNA。它通过靶向MAKP6抑制肿瘤生长。新近确定的信号转导轴可以作为治疗宫颈癌的新型治疗靶标。敲除MAPK6可抑制子​​宫颈癌细胞的增殖，迁移和侵袭能力。我们的研究是首次将miR-144-3p报告为宫颈癌的抑癌miRNA。它通过靶向MAKP6抑制肿瘤生长。新近确定的信号转导轴可以作为治疗宫颈癌的新型治疗靶标。敲除MAPK6可抑制子​​宫颈癌细胞的增殖，迁移和侵袭能力。我们的研究是首次将miR-144-3p报告为宫颈癌的抑癌miRNA。它通过靶向MAKP6抑制肿瘤生长。新近确定的信号转导轴可以作为治疗宫颈癌的新型治疗靶标。