Aberrant activation of hepatocyte growth factor/scatter factor (HGF/SF) and its receptor, Met, is involved in the development and progression of many human cancers. In the screening assay of extracts from the root tuber of Tetrastigma hemsleyanum Diels et Gilg, isoquercitrin inhibited HGF/SF-Met signaling as indicated by its inhibitory activity on HGF/SF-induced cell scattering. Further analysis revealed that isoquercitrin specifically inhibited HGF/SF-induced tyrosine phosphorylation of Met. We also found that isoquercitrin decreased HGF-induced migration and invasion by parental or HGF/SF-transfected bladder carcinoma cell line NBT-II cells. Furthermore, isoquercitrin inhibited HGF/SF-induced epithelial mesenchymal transition in vitro and the invasion/metastasis of HGF/SF-transfected NBT-II cells in vivo. Our data suggest the possible use of isoquercitrin in human cancers associated with dysregulated HGF/SF-Met signaling.

中文翻译：

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异槲皮苷是四角叉菜中的成分，可抑制肝细胞生长因子/分散因子诱导的肿瘤细胞迁移和侵袭。

肝细胞生长因子/散射因子（HGF / SF）及其受体Met的异常激活与许多人类癌症的发生和发展有关。在对四叶草（Tetrastigma hemsleyanum Diels et Gilg）的块根提取物的筛选测定中，异槲皮苷抑制HGF / SF-Met信号传导，如其对HGF / SF诱导的细胞散射的抑制活性所示。进一步的分析表明，异槲皮苷能特异性抑制HGF / SF诱导的Met酪氨酸磷酸化。我们还发现异槲皮苷降低了HGF诱导的亲代或HGF / SF转染的膀胱癌细胞系NBT-II细胞的迁移和侵袭。此外，异槲皮苷在体外抑制HGF / SF诱导的上皮间充质转化以及在体内抑制HGF / SF转染的NBT-II细胞的侵袭/转移。