Germination is the first step of plant growth in plant life cycle. An embryonic radicle protruding the seed coat is the first part of plant which has direct contact with external environment including salt-affected soil. In embryo axes, mitochondria are the main energy producer. To understand better salinity impact on mitochondria functioning, this study was focused on the effect of NaCl stress onto mitochondria proteome. Mitochondria were isolated from yellow lupine (Lupine luteus L. 'Mister') embryo axes cultured in vitro for 12 h with 250 and 500 mM NaCl. Two-dimensional gel electrophoresis of mitochondrial proteins isolated from NaCl-treated axes demonstrated significant changes in proteins abundances as a response to salinity treatment. Twenty-one spots showing significant changes in protein expression profiles both under 250 and 500 mM NaCl treatment were selected for tandem mass spectrometry identification. This approach revealed proteins associated with different metabolic processes that represent enzymes of tricarboxylic acid cycle, mitochondrial electron transport chain, enzymes and proteins involved in mitochondria biogenesis and stresses response. Among proteins involved in mitochondria biogenesis, mitochondrial import inner membrane translocase, subunit Tim17/22, mitochondrial-processing peptidase subunit alpha-1, mitochondrial elongation factor Tu and chaperonins CPN60 were revealed. Finally, formate dehydrogenase 1 was found to accumulate in lupine embryo axes mitochondria under salinity. The functions of identified proteins are discussed in relation to salinity stress response, including salinity-induced PCD.

中文翻译：

---

羽扇豆胚轴在盐胁迫下。二。线粒体蛋白质组反应。

发芽是植物生命周期中植物生长的第一步。从种皮上伸出的胚根是植物的第一部分，它直接与外部环境（包括受盐影响的土壤）接触。在胚胎轴中，线粒体是主要的能量产生者。为了了解更好的盐度对线粒体功能的影响，本研究的重点是NaCl胁迫对线粒体蛋白质组的影响。从黄色羽扇豆（羽扇豆Luteus L.'Mister'）胚轴分离线粒体，体外用250和500 mM NaCl培养12小时。从NaCl处理的轴分离出的线粒体蛋白质的二维凝胶电泳显示，作为对盐度处理的响应，蛋白质丰度发生了显着变化。选择在250和500 mM NaCl处理下显示蛋白质表达谱显着变化的21个斑点进行串联质谱鉴定。这种方法揭示了与不同代谢过程相关的蛋白质，这些蛋白质代表三羧酸循环的酶，线粒体电子传输链，参与线粒体生物发生并强调反应的酶和蛋白质。在涉及线粒体生物发生的蛋白质中，线粒体导入内膜转座酶，Tim17 / 22亚基，线粒体加工肽酶亚基α-1，线粒体延伸因子Tu和伴侣蛋白CPN60被揭示。最后，在盐度下，甲酸脱氢酶1积累在羽扇豆的胚轴线粒体中。