The soft rot Enterobacteriaceae (SRE) Pectobacterium and Dickeya species (formerly classified as pectinolytic Erwinia spp.) cause important diseases on potato and other arable and horticultural crops. They may affect the growing potato plant causing blackleg and are responsible for tuber soft rot in storage thereby reducing yield and quality. Efficient and cost-effective detection and identification methods are essential to investigate the ecology and pathogenesis of the SRE as well as in seed certification programmes. The aim of this review was to collect all existing information on methods available for SRE detection. The review reports on the sampling and preparation of plant material for testing and on over thirty methods to detect, identify and differentiate the soft rot and blackleg causing bacteria to species and subspecies level. These include methods based on biochemical characters, serology, molecular techniques which rely on DNA sequence amplification as well as several less-investigated ones.

中文翻译：

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引起马铃薯黑胫病和块茎软腐病的果杆菌属和迪克杆菌属的检测、鉴定和区分：综述


软腐肠杆菌科 (SRE) 果杆菌属和迪克氏菌属（以前被归类为解果胶欧文氏菌属）会引起马铃薯和其他耕地和园艺作物的重要疾病。它们可能会影响正在生长的马铃薯植株，导致黑胫病，并导致储存中的块茎软腐病，从而降低产量和质量。高效且具有成本效益的检测和识别方法对于研究 SRE 的生态和发病机制以及种子认证计划至关重要。本次审查的目的是收集有关可用于 SRE 检测的方法的所有现有信息。该审查报告了用于测试的植物材料的取样和制备，以及检测、识别和区分导致软腐病和黑胫病的细菌到物种和亚种水平的三十多种方法。其中包括基于生化特征、血清学、依赖 DNA 序列扩增的分子技术以及一些研究较少的方法。