Elevated Pb levels in humans through environmental exposure are a significant health concern requiring scientific study of the sources of, and physiological response to this toxin. This requires a simple and precise method for measuring radiogenic Pb isotopes and Pb levels in blood. Presented here is a combination of methods for separation and analysis of Pb previously used predominantly for geologic samples. This includes separation of Pb from the complex matrix of blood samples using an Fe co-precipitation method, followed by isotopic analysis by multi-collector inductively coupled plasma mass spectrometry. Evaluation of the efficacy of this procedure shows that the precision of sample preparations as measured by % difference between the (207)Pb/(206)Pb of duplicate analyses averages 0.064% (n = 48). Using the same preparation and analysis techniques to measure Pb concentrations by isotope dilution resulted in a reproducibility of better than 6%. The method was successfully used to measure uptake of ingested soil Pb in a study of the bioavailability of Pb in contaminated soils.

中文翻译：

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通过 Fe 共沉淀和 MC-ICPMS 分析快速、精确地测定全血中的 Pb 同位素

环境暴露导致人体铅含量升高是一个重要的健康问题，需要对这种毒素的来源和生理反应进行科学研究。这需要一种简单而精确的方法来测量血液中的放射性铅同位素和铅水平。这里介绍的是以前主要用于地质样品的铅分离和分析方法的组合。这包括使用 Fe 共沉淀方法从血液样本的复杂基质中分离 Pb，然后通过多接收器电感耦合等离子体质谱法进行同位素分析。对该程序有效性的评估表明，通过重复分析的 (207)Pb/(206)Pb 之间的百分比差异测量的样品制备精度平均为 0.064% (n = 48)。使用相同的制备和分析技术通过同位素稀释测量 Pb 浓度，重现性优于 6%。在研究受污染土壤中铅的生物有效性时，该方法成功地用于测量摄入的土壤铅的吸收。