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Morphological rearrangement of the cortical region, in aging ovaries.
Histology and Histopathology ( IF 2.5 ) Pub Date : 2018-12-27 , DOI: 10.14670/hh-18-078
Verónica Díaz-Hernández 1 , Ivette Caldelas 2 , Luis M Montaño 3 , Horacio Merchant-Larios 2
Affiliation  

The ovary is a structurally dynamic organ that alters with age. Modifications in the paracrine status influence the capacity of aging oocytes to develop normal embryos. Despite the importance of understanding the cellular and molecular mechanism involved in the process of ovarian aging, histological changes remain poorly understood. Correlating the process of folliculogenesis and somatic cell function during ovarian aging is essential to explain the reproductive decline of aged mammalian species, including humans. Here, we performed a morphological and immunohistological study on the ovaries of chinchilla rabbits that varied in age from one to 34-months. The spatiotemporal expression of the cholesterol side-chain cleavage cytochrome P450scc (CYP11A) and the smooth muscle actin (SMA) were analyzed. A significant histological rearrangement of immunodetected cells in theca interna, theca externa and the interstitial tissue around the follicles occurred. The expression of CYP11A1 decreased considerably in antral follicles of aging ovaries. Moreover, we found that the secondary interstitial gland developed extensively, and a remarkable rearrangement of the surface epithelium occurred in aging ovaries. In contrast to ovaries during the reproductive period, the immunohistological changes demonstrate that the interstitial gland became the most abundant tissue during the aging of ovaries. Thus, the current study provides new data for understanding the alteration of somatic cell function in elderly ovaries and how this affects their declined fertility.

中文翻译:

卵巢老化中皮质区域的形态重排。

卵巢是随年龄变化的结构动态器官。旁分泌状态的改变影响衰老的卵母细胞发育正常胚胎的能力。尽管了解卵巢衰老过程中涉及的细胞和分子机制很重要,但对组织学变化的了解仍然很少。在卵巢衰老过程中,将卵泡形成过程与体细胞功能相关联对于解释包括人类在内的衰老哺乳动物物种的生殖衰退至关重要。在这里,我们对年龄从1个月到34个月不等的栗鼠兔子的卵巢进行了形态学和免疫组织学研究。分析了胆固醇侧链裂解细胞色素P450scc(CYP11A)和平滑肌肌动蛋白(SMA)的时空表达。发生在国际大肠膜,外大肠膜和卵泡周围的间质组织中的免疫检测细胞发生了明显的组织学重排。CYP11A1在卵巢衰老的卵泡中表达明显下降。此外,我们发现次级间质腺广泛发展,并且在衰老的卵巢中发生了表面上皮的显着重排。与生殖期的卵巢相反,免疫组织学变化表明,在卵巢衰老过程中,间质腺成为最丰富的组织。因此,当前的研究为理解老年卵巢中体细胞功能的变化及其对生育能力下降的影响提供了新的数据。发生外囊和卵泡周围的间质组织。CYP11A1在卵巢衰老的卵泡中表达明显下降。此外,我们发现次级间质腺广泛发展,并且在衰老的卵巢中发生了表面上皮的显着重排。与生殖期的卵巢相反,免疫组织学变化表明,在卵巢衰老过程中,间质腺成为最丰富的组织。因此,当前的研究为理解老年卵巢中体细胞功能的变化及其对生育能力下降的影响提供了新的数据。发生外囊和卵泡周围的间质组织。CYP11A1在卵巢衰老的卵泡中表达明显下降。此外,我们发现次级间质腺广泛发展,并且在衰老的卵巢中发生了表面上皮的显着重排。与生殖期的卵巢相反,免疫组织学变化表明,在卵巢衰老过程中,间质腺成为最丰富的组织。因此,当前的研究为理解老年卵巢中体细胞功能的变化及其对生育能力下降的影响提供了新的数据。并在衰老的卵巢中发生明显的表面上皮重排。与生殖期的卵巢相反,免疫组织学变化表明,在卵巢衰老过程中,间质腺成为最丰富的组织。因此,当前的研究为理解老年卵巢中体细胞功能的变化及其对生育能力下降的影响提供了新的数据。并在衰老的卵巢中发生明显的表面上皮重排。与生殖期的卵巢相反,免疫组织学变化表明,在卵巢衰老过程中,间质腺成为最丰富的组织。因此,当前的研究为理解老年卵巢中体细胞功能的变化及其对生育能力下降的影响提供了新的数据。
更新日期:2020-08-21
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