Isotretinoin is an analogue of vitamin A and by suppressing the sebaceous glands it is often prescribed in cases of severe acne treatment. The treatment for the average patient is carried out during two to ten months. This study was designed to investigate liver structure, hepatic enzyme levels and the stress oxidative parameter after isotretinoin treatment during a similar period and using the dosages of 1 mg/kg and another one of 10 mg/kg in young male Wistar rats. We have analyzed the blood serum biochemical levels to determine hepatic function and lipid peroxidation, hepatic tissue levels of hepatic enzymes, histology and ultrastructure. The groups receiving 1 mg/kg were not altered after treatment. Their ultrastructure showed a metabolically more active organ after treatment with 10 mg/kg, in which there was an increase in the area occupied by mitochondria and rough reticulum in electron transmission images. The group that received 10 mg/kg also showed increased alkaline phosphatase, decreased high density lipoprotein and low density lipoprotein. The changes observed with the 10 mg/kg dose were not conclusive for liver damage, because of the lack of histological structural modifications and the few biochemical alterations. The 1 mg/kg dose showed a liver responding to some stimuli but without profound alterations. So, we confirm that the proposed protocol with 1mg/kg or 10 mg/kg isotretinoin did not cause important biochemical and histological disfunctions for male Wistar rat livers.

中文翻译：

---

通过肝脏参数测量异维A酸的安全性。

异维A酸是维生素A的类似物，通过抑制皮脂腺，经常在严重痤疮治疗中开处方。对普通患者的治疗在两到十个月内进行。这项研究旨在研究异维A酸治疗后相似时期的肝脏结构，肝酶水平和应激氧化参数，并在年轻雄性Wistar大鼠中使用1 mg / kg的剂量和10 mg / kg的另一种剂量。我们已经分析了血清生化水平，以确定肝功能和脂质过氧化，肝组织中肝酶水平，组织学和超微结构。接受1 mg / kg治疗的组在治疗后没有改变。经10 mg / kg处理后，它们的超微结构显示出代谢活性更高的器官，在电子透射图像中，线粒体和粗糙的网状结构所占的面积增加了。接受10 mg / kg的组还显示出碱性磷酸酶增加，高密度脂蛋白和低密度脂蛋白减少。由于缺乏组织学结构修饰和少量生化改变，因此以10 mg / kg剂量观察到的变化不是肝损伤的结论。1 mg / kg剂量显示肝脏对某些刺激有反应，但无明显变化。因此，我们确认拟议的1mg / kg或10mg / kg异维A酸的方案不会对雄性Wistar大鼠肝脏造成重要的生化和组织功能异常。降低高密度脂蛋白和低密度脂蛋白。由于缺乏组织学结构修饰和少量生化改变，因此以10 mg / kg剂量观察到的变化不是肝损伤的结论。1 mg / kg剂量显示肝脏对某些刺激有反应，但无明显变化。因此，我们确认拟议的1mg / kg或10mg / kg异维A酸的方案不会对雄性Wistar大鼠肝脏造成重要的生化和组织功能异常。降低高密度脂蛋白和低密度脂蛋白。由于缺乏组织学结构修饰和少量生化改变，因此以10 mg / kg剂量观察到的变化不是肝损伤的结论。1 mg / kg剂量显示肝脏对某些刺激有反应，但无明显变化。因此，我们确认拟议的1mg / kg或10mg / kg异维A酸的方案不会对雄性Wistar大鼠肝脏造成重要的生化和组织功能异常。