HIPK2 is an evolutionarily conserved serine/threonine kinase and is considered a co-regulator of an increasing number of transcription factors modulating a variety of cellular processes, including inflammation, proliferation and fibrosis. Skeletal muscle injuries repair is an overlapping event between inflammation and tissue repair. There are no reports about HIPK2 expression in skeletal muscles after trauma. A foundational study on distribution and time-dependent expression of HIPK2 was performed by immunohistochemical staining, Western blotting and quantitative real-time PCR, which is expected to obtain a preliminary insight into the functions of HIPK2 during the repair of contused skeletal muscle in mice. An animal model of skeletal muscle contusion was established in 50 C57B6/L male mice. Samples were taken at 1, 3, 5, 7, 9, 14, 17, 21 and 28 days after contusion, respectively (5 mice at each posttraumatic interval). 5 mice were employed as control. No HIPK2-positive staining was detected in uninjured skeletal muscle. Intensive immunoreactivties of HIPK2 were observed in polymorphonuclear cells, round-shaped mononuclear cells, regenerated multinucleated myotubes and spindle-shaped fibroblastic cells in the contused tissue. The HIPK2-positive cells were identified as neutrophils, macrophages and myofibroblasts by double immunofluorescent procedure. HIPK2 protein and mRNA expression were remarkably up-regulated after contusion by Western blotting and qPCR analysis. The results demonstrated that the expression of HIPK2 is distributed in certain cell types and is time-dependently expressed in skeletal muscle after contusion, which suggested that HIPK2 may participate in the whole process of skeletal muscle wound healing, including inflammatory response, muscle regeneration and fibrogenesis.

中文翻译：

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HIPK2在小鼠骨骼肌挫伤修复过程中的分布和时间依赖性表达。

HIPK2是一种进化上保守的丝氨酸/苏氨酸激酶，被认为是调节多种细胞过程（包括炎症，增殖和纤维化）的转录因子数量增加的共调节因子。骨骼肌损伤修复是炎症和组织修复之间的重叠事件。没有关于创伤后骨骼肌中HIPK2表达的报道。通过免疫组织化学染色，Western印迹和定量实时PCR对HIPK2的分布和时间依赖性表达进行了基础研究，有望初步了解HIPK2在小鼠骨骼肌挫伤修复中的功能。在50只C57B6 / L雄性小鼠中建立了骨骼肌挫伤的动物模型。在1、3、5、7、9、14、17采样 挫伤后分别为21天和28天（每个创伤后间隔为5只小鼠）。将5只小鼠用作对照。在未受伤的骨骼肌中未检测到HIPK2阳性染色。在挫伤组织中的多形核细胞，圆形单核细胞，再生的多核肌管和纺锤形成纤维细胞中观察到了HIPK2的强免疫反应性。通过双重免疫荧光法将HIPK2阳性细胞鉴定为嗜中性粒细胞，巨噬细胞和成肌纤维细胞。Western blotting和qPCR分析显示挫伤后HIPK2蛋白和mRNA表达明显上调。结果表明，HIPK2的表达分布在某些细胞类型中，在挫伤后骨骼肌中呈时间依赖性表达，