Different regions of the mammalian nose smell different odors. In the mouse olfactory system, spatially regulated expression of >1000 olfactory receptors (ORs) along the dorsomedial-ventrolateral (DV) axis forms a topological map in the main olfactory epithelium (MOE). However, the locations of most ORs along the DV axis are currently unknown. By sequencing mRNA of 12 isolated MOE pieces, we mapped out the DV locations-as quantified by "zone indices" on a scale of 1-5-of 1033 OR genes with an estimated error of 0.3 zone indices. Our map covered 81% of all intact OR genes and 99.4% of the total OR mRNA abundance. Spatial regulation tended to vary gradually along chromosomes. We further identified putative non-OR genes that may exhibit spatial expression along the DV axis.

中文翻译：

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小鼠主嗅上皮中嗅觉受体的近乎完整的空间图。


哺乳动物鼻子的不同区域闻到不同的气味。在小鼠嗅觉系统中，>1000 嗅觉受体 (OR) 沿背内侧-腹外侧 (DV) 轴的空间调节表达在主嗅上皮 (MOE) 中形成拓扑图。然而，大多数 OR 沿 DV 轴的位置目前未知。通过对 12 个分离的 MOE 片段的 mRNA 进行测序，我们绘制了 1033 个 OR 基因的 DV 位置（按 1-5 级的“区域指数”量化），估计误差为 0.3 个区域指数。我们的图谱覆盖了所有完整 OR 基因的 81% 和总 OR mRNA 丰度的 99.4%。空间调节往往沿着染色体逐渐变化。我们进一步鉴定了可能沿 DV 轴表现出空间表达的推定非 OR 基因。