Different regions of the mammalian nose smell different odors. In the mouse olfactory system, spatially regulated expression of >1000 olfactory receptors (ORs) along the dorsomedial-ventrolateral (DV) axis forms a topological map in the main olfactory epithelium (MOE). However, the locations of most ORs along the DV axis are currently unknown. By sequencing mRNA of 12 isolated MOE pieces, we mapped out the DV locations-as quantified by "zone indices" on a scale of 1-5-of 1033 OR genes with an estimated error of 0.3 zone indices. Our map covered 81% of all intact OR genes and 99.4% of the total OR mRNA abundance. Spatial regulation tended to vary gradually along chromosomes. We further identified putative non-OR genes that may exhibit spatial expression along the DV axis.

中文翻译：

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小鼠主要嗅觉上皮细胞中嗅觉受体的近乎完整空间图。

哺乳动物鼻子的不同区域闻到不同的气味。在小鼠嗅觉系统中，沿背侧-腹侧（DV）轴的> 1000个嗅觉受体（OR）的空间调控表达在主要嗅觉上皮（MOE）中形成拓扑图。但是，大多数OR沿DV轴的位置目前未知。通过对12个分离的MOE片段的mRNA进行测序，我们以1033个OR基因中的1至5个比例绘制了DV位置（由“区域指数”量化），估计误差为0.3个区域指数。我们的图谱涵盖了所有完整OR基因的81％和OR mRNA总量的99.4％。空间调节趋向于沿着染色体逐渐变化。我们进一步确定了可能显示沿DV轴空间表达的非OR基因。