Encephalitozoon spp. is an obligate intracellular microsporidian parasite that infects a wide range of mammalian hosts, including humans. This study was conducted to determine the prevalence of Encephalitozoon spp. in wild living rodents from Poland, the Czech Republic and Slovakia. Faecal and spleen samples were collected from individuals of Apodemus agrarius, Apodemus flavicollis, Apodemus sylvaticus, and Myodes glareolus (n = 465) and used for DNA extraction. PCR, targeting the ITS region of the rRNA gene was performed. The overall prevalence of microsporidia was 15.1%. The occurrence of Encephalitozoon cuniculi in the abovementioned host species of rodents has been presented for the first time, with the highest infection rate recorded for A. flavicollis. Sequence analysis showed that the most frequent species was E. cuniculi genotype II (92.5%). E. cuniculi genotypes I (1.5%) and III (6.0%) were also identified.

脑虫属 是专一的细胞内微孢子虫寄生虫，可感染包括人类在内的多种哺乳动物宿主。进行了这项研究，以确定脑炎菌的流行程度。在来自波兰，捷克共和国和斯洛伐克的野生活体啮齿动物中。粪便和脾样品从个人收集的黑线姬鼠，姬flavicollis，姬鼠，和Myodes glareolus（N = 465）并用于DNA提取。靶向rRNA基因的ITS区域的PCR被执行。小孢子虫病的总体患病率为15.1％。头状脑病的发生在上述啮齿动物的宿主物种中，这是首次出现，其中黄曲霉的感染率最高。序列分析表明，最常见的物种是cu.cuniculi基因型II（92.5％）。还鉴定到了大肠杆菌（E. cuniculi）基因型I（1.5％）和III。（III）（6.0％）。