INTRODUCTION The objective of this study was to assess the performance of a technique (S. PneumoStrip test) based on PCR followed by reverse strip hybridisation for the detection of Streptococcus pneumoniae serotypes directly in blood culture vials. METHODS One hundred and ten (110) pairs of isolated strains and their corresponding original blood cultures vials were studied in parallel. Pure isolated strains were conventionally serotyped using latex agglutination and the Quellung reaction. The S. PneumoStrip test was carried out directly in the original blood culture samples. RESULTS In 102 cases (92.7%), results of the serotype obtained by Quellung coincided with their corresponding original blood cultures typed by S. PneumoStrip. CONCLUSIONS S. PneumoStrip test is a good alternative technique for direct pneumococcal serotyping in blood culture clinical samples.

中文翻译：

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通过 PCR 反向杂交技术直接鉴定血培养物中的肺炎球菌血清型。

引言 本研究的目的是评估基于 PCR 和反向条带杂交的技术（S. PneumoStrip 测试）的性能，以直接检测血培养瓶中的肺炎链球菌血清型。方法平行研究一百一十 (110) 对分离菌株及其相应的原始血培养瓶。使用乳胶凝集和 Quellung 反应对纯分离菌株进行常规血清分型。S. PneumoStrip 测试直接在原始血培养样品中进行。结果 102例（92.7%），Quellung获得的血清型结果与其相应的S.PneumoStrip原始血培养结果一致。结论 S。