Background Campylobacter jejuni is the leading cause of bacterial gastroenteritis (campylobacteriosis) in humans worldwide, and the most frequent pathogen associated with Guillain-Barré syndrome (GBS) and Miller-Fisher syndrome (MFS). The study was designed in order to assess similarities between genomes of Campylobacter jejuni strains, isolated from children suffering from acute diarrhea in northeastern Poland, in comparison to C. jejuni genomes stored in public databases. The analysis involved phylogeny, resistome and virulome. In addition, the Campylobacter PubMLST database was used to estimate the prevalence of the analyzed C. jejuni sequence type (STs) in other countries. Results Campylobacter jejuni ST50, ST257 and ST51 represented 5.3%, 4.5% and 2.2% of the PubMLST records, respectively. Overall, strains representing the STs showed common resistance to tetracyclines (51.3%) and fluoroquinolones (31.8%), mediated through the tetO gene (98.2%) and point mutation (T86I) in the gyrA gene (100%). However, the latter was present in all our isolates. The major differences in virulence patterns concerned serotypes, lipooligosaccharide (LOS) classes and certain clinically relevant genes. Conclusions Campylobacter jejuni ST50, ST51 and ST257 are among the top ten of STs isolated in Europe. WGS revealed diversity of serotypes and LOS classes in ST50 strains, that deserves further clinical and epidemiological investigations as it might be related to a risk of post-infectious neurological sequels such as Guillain-Barré syndrome. Additionally, the results implicate lower pathogenic potential and distinct transmission chains or reservoirs for C. jejuni ST51 isolates responsible for campylobacteriosis in northeastern Poland.

中文翻译：

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从波兰东北部腹泻患者中分离出的空肠弯曲杆菌菌株的全基因组比较分析。

背景空肠弯曲杆菌是全世界人类细菌性胃肠炎（弯曲杆菌病）的主要原因，也是与格林-巴利综合征（GBS）和米勒-费舍尔综合征（MFS）相关的最常见病原体。该研究旨在评估从波兰东北部患有急性腹泻的儿童中分离出来的空肠弯曲杆菌菌株的基因组与存储在公共数据库中的空肠弯曲杆菌基因组之间的相似性。分析涉及系统发育、抗性组和病毒组。此外，Campylobacter PubMLST 数据库用于估计所分析的空肠弯曲杆菌序列类型 (STs) 在其他国家的流行率。结果 空肠弯曲杆菌 ST50、ST257 和 ST51 分别占 PubMLST 记录的 5.3%、4.5% 和 2.2%。总体，代表 ST 的菌株对四环素类药物 (51.3%) 和氟喹诺酮类药物 (31.8%) 表现出共同的耐药性，通过 tetO 基因 (98.2%) 和 gyrA 基因中的点突变 (T86I) (100%) 介导。然而，后者存在于我们所有的分离物中。毒力模式​​的主要差异涉及血清型、脂寡糖 (LOS) 类别和某些临床相关基因。结论空肠弯曲杆菌ST50、ST51和ST257在欧洲分离出的STs中位居前10位。WGS 揭示了 ST50 菌株中血清型和 LOS 类别的多样性，这值得进一步的临床和流行病学调查，因为它可能与感染后神经系统后遗症（如格林-巴利综合征）的风险有关。此外，结果表明 C.