Currently vitrification is the method of choice for low-temperature preservation of oocytes and embryos. However, that was not the case until about 10 years ago when freezing methods were used relatively successfully for embryos and investigated (unsuccessfully) for oocyte preservation. In this paper we will review the history of oocyte and embryo cryopreservation and look into ways and methods for overcoming and improving the vitrification method since it suffers from inherent disadvantages since it is a cumbersome, time-consuming and costly procedure.

中文翻译：

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卵母细胞和胚胎玻璃化冷冻的近期前景：回顾过去的经验和规划未来

目前玻璃化冷冻是卵母细胞和胚胎低温保存的首选方法。然而，直到大约 10 年前，冷冻方法相对成功地用于胚胎并研究（不成功）卵母细胞保存时，情况才如此。在本文中，我们将回顾卵母细胞和胚胎冷冻保存的历史，并探讨克服和改进玻璃化冷冻方法的方法和方法，因为它具有固有的缺点，因为它是一个繁琐、耗时和昂贵的过程。