Gold nanoparticles (Au NPs) hold great promise in food, industrial and biomedical applications due to their unique physicochemical properties. However, influences of the gastrointestinal tract (GIT), a likely route for Au NPs administration, on the physicochemical properties of Au NPs has been rarely evaluated. Here, we investigated the influence of GIT fluids on the physicochemical properties of Au NPs (5, 50, and 100 nm) and their implications on intestinal epithelial permeability in vitro. Au NPs aggregated in fasted gastric fluids and generated hydroxyl radicals in the presence of H2O2. Cell studies showed that GIT fluids incubation of Au NPs affected the cellular uptake of Au NPs but did not induce cytotoxicity or disturb the intestinal epithelial permeability.

中文翻译：

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模拟胃肠道环境对金纳米颗粒理化性质的影响及其对肠上皮通透性的影响。

金纳米颗粒（Au NPs）具有独特的理化特性，在食品，工业和生物医学应用中具有广阔的前景。然而，很少评估胃肠道（GIT）对Au NPs的理化性质的影响。在这里，我们调查了GIT液对Au NPs（5、50和100 nm）的理化特性的影响及其对体外肠上皮渗透性的影响。Au NPs在禁食的胃液中聚集并在H2O2存在下产生羟基自由基。细胞研究表明，GIT液对Au NPs的孵育影响了Au NPs的细胞摄取，但没有诱导细胞毒性或干扰肠道上皮通透性。