OBJECTIVE To investigate the expression of miR-21, miR-221, miR-143, and miR-106a in patients' osteosarcoma samples, and to explore the correlation between these microRNAs (miRNAs) and the clinical stage of osteosarcoma. METHODS RNA was extracted from tumor and tumor-adjacent normal bone tissues from 94 patients with osteosarcoma. RNA reverse-transcription was carried out using an miRNA reverse transcription kit. The levels of miR-21, miR-221, miR-143, and miR-106a in osteosarcoma and normal bone tissues were analyzed by real-time polymerase chain reaction using SYBR Premix Ex Taq™II. RESULTS The expression levels of miR-21, mirR-221, and miR-106a were significantly higher in 90.42%, 84.04%, and 92.55 % of the osteosarcoma samples compared to the adjacent normal tissues (P<0.05), respectively. While the expression of miR-143 was significantly lower compared to the adjacent normal tissues (P <0.05). Moreover, the expression levels of miR-21 and miR-221 were positively correlated with the Enneking clinical stage and the presence of lung metastasis (P <0.05), while the expression levels of miR-143 and miR-106a showed a significant inverse and direct correlation respectively, with the tumor grade. CONCLUSIONS The upregulation of miR-21, miR-221, and miR-106a, as well as the down-regulation of miR-143 were correlated with the pathological stage, tumor grade, and lung metastasis. Therefore, the levels of these miRNAs can serve as potential biomarkers for the early diagnosis of osteosarcoma, and can be used as potential therapeutic targets.

中文翻译：

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肿瘤 miR-21、miR-221、miR-143 和 miR-106a 作为骨肉瘤患者生物标志物的临床意义。

目的研究患者骨肉瘤标本中miR-21、miR-221、miR-143和miR-106a的表达情况，探讨这些microRNAs（miRNAs）与骨肉瘤临床分期的相关性。方法 从 94 例骨肉瘤患者的肿瘤和与肿瘤相邻的正常骨​​组织中提取 RNA。使用miRNA逆转录试剂盒进行RNA逆转录。使用 SYBR Premix Ex Taq™II 通过实时聚合酶链反应分析骨肉瘤和正常骨组织中 miR-21、miR-221、miR-143 和 miR-106a 的水平。结果 90.42%、84.04%和92.55%的骨肉瘤标本中miR-21、mirR-221和miR-106a的表达水平显着高于邻近正常组织（P<0.05）。而与邻近正常组织相比，miR-143的表达显着降低（P <0.05）。此外，miR-21和miR-221的表达水平与Enneking临床分期和肺转移的存在呈正相关（P <0.05），而miR-143和miR-106a的表达水平呈显着负相关，分别与肿瘤分级直接相关。结论 miR-21、miR-221和miR-106a的上调以及miR-143的下调与病理分期、肿瘤分级和肺转移有关。因此，这些miRNA的水平可以作为骨肉瘤早期诊断的潜在生物标志物，并可以作为潜在的治疗靶点。miR-21和miR-221的表达水平与Enneking临床分期和肺转移的存在呈正相关（P <0.05），而miR-143和miR-106a的表达水平呈显着负相关和正相关分别与肿瘤分级。结论 miR-21、miR-221和miR-106a的上调以及miR-143的下调与病理分期、肿瘤分级和肺转移有关。因此，这些miRNA的水平可以作为骨肉瘤早期诊断的潜在生物标志物，并可以作为潜在的治疗靶点。miR-21和miR-221的表达水平与Enneking临床分期和肺转移的存在呈正相关（P <0.05），而miR-143和miR-106a的表达水平呈显着负相关和正相关分别与肿瘤分级。结论 miR-21、miR-221和miR-106a的上调以及miR-143的下调与病理分期、肿瘤分级和肺转移有关。因此，这些miRNA的水平可以作为骨肉瘤早期诊断的潜在生物标志物，并可以作为潜在的治疗靶点。而miR-143和miR-106a的表达水平分别与肿瘤分级呈显着负相关和正相关。结论 miR-21、miR-221和miR-106a的上调以及miR-143的下调与病理分期、肿瘤分级和肺转移有关。因此，这些miRNA的水平可以作为骨肉瘤早期诊断的潜在生物标志物，并可以作为潜在的治疗靶点。而miR-143和miR-106a的表达水平分别与肿瘤分级呈显着负相关和正相关。结论 miR-21、miR-221和miR-106a的上调以及miR-143的下调与病理分期、肿瘤分级和肺转移有关。因此，这些miRNA的水平可以作为骨肉瘤早期诊断的潜在生物标志物，并可以作为潜在的治疗靶点。