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Characterizing the Effect of Automated Cell Sorting Solutions on Cytomorphological Changes.
Acta Cytologica ( IF 1.6 ) Pub Date : 2019-06-24 , DOI: 10.1159/000500769
Katsuhide Ikeda 1 , Shouichi Sato 2 , Hiroshi Chigira 3 , Yasuo Shibuki 3 , Nobuyoshi Hiraoka 3
Affiliation  

INTRODUCTION Liquid-based cytology has become a widely adopted, automated screening system for gynecologic and nongynecologic cytology. Automated screening systems function by distinguishing atypical cells based on their cytoplasmic and nuclear areas, densitometric measurement, and so on. However, the morphological influence of the washing solution has not been fully considered. Here, we examined the morphological effect and temporal change resulting from saving the cytologic samples in various solutions. METHODS Cytologic specimens were obtained from the ascites (AS) of patients with peritoneal cancer. Various solutions of a physiological saline, a Ringer's solution, a low-molecular dextran L injection, VOLUVEN 6% solution, MIXID L injection (ML), RPMI-1640 medium, and horse serum (HS) were added to aliquot sediments. All samples were refrigerated at 4°C, and aliquots were subsequently processed at specific time points (0, 1, 2, 4, 7, and 14 days). For all samples, cytoplasmic and nuclear size of the Papanicolaou-stained specimens were measured. RESULTS In terms of cytoplasmic and nuclear areas, samples stored in ML and HS showed no significant difference compared to the AS sample; in contrast, the other samples were significantly larger in both cytoplasmic and nuclear areas than the AS sample. In examining the temporal change among the solutions, we found that the cytoplasms and nuclei became small over the time course for all of the tested solutions. CONCLUSION We showed that cells swell in the solution after 1 h of storage and contract as time progresses. Together, our findings have important implications for how mathematical analysis is applied during the automated screening process.

中文翻译:

表征自动细胞分选溶液对细胞形态变化的影响。

引言液基细胞学已成为一种广泛用于妇科和非妇科细胞学的自动筛选系统。自动化筛选系统的功能是根据非典型细胞的胞质和核区域,光密度测定法等来对其进行区分。但是,尚未充分考虑洗涤溶液的形态学影响。在这里,我们研究了将细胞学样品保存在各种溶液中后产生的形态学效应和时间变化。方法从腹膜癌患者的腹水(AS)中获取细胞学标本。将生理盐水,林格氏溶液,低分子右旋糖酐L注射液,VOLUVEN 6%溶液,MIXID L注射液(ML),RPMI-1640培养基和马血清(HS)的各种溶液添加到等分沉淀物中。将所有样品在4°C冷藏,然后在特定时间点(0、1、2、4、7和14天)处理等分试样。对于所有样品,均测量了巴氏染色的标本的细胞质和核大小。结果在细胞质和核面积方面,ML和HS中保存的样品与AS样品相比无显着差异。相反,其他样品在细胞质和核区域均显着大于AS样品。在检查溶液之间的时间变化时,我们发现所有测试溶液的细胞质和细胞核都随着时间的流逝而变小。结论我们显示,在储存1小时后,细胞在溶液中会膨胀并随着时间的推移而收缩。一起,
更新日期:2019-11-01
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