BACKGROUND The fast-moving progress of the third-generation long-read sequencing technologies will soon bring the biological and medical sciences to a new era of research. Altogether, the technique and experimental procedures are becoming more straightforward and available to biologists from diverse fields, even without any profound experience in DNA sequencing. Thus, the introduction of the MinION device by Oxford Nanopore Technologies promises to "bring sequencing technology to the masses" and also allows quick and operative analysis in field studies. However, the convenience of this sequencing technology dramatically contrasts with the available analysis tools, which may significantly reduce enthusiasm of a "regular" user. To really bring the sequencing technology to every biologist, we need a set of user-friendly tools that can perform a powerful analysis in an automatic manner. FINDINGS NanoPipe was developed in consideration of the specifics of the MinION sequencing technologies, providing accordingly adjusted alignment parameters. The range of the target species/sequences for the alignment is not limited, and the descriptive usage page of NanoPipe helps a user to succeed with NanoPipe analysis. The results contain alignment statistics, consensus sequence, polymorphisms data, and visualization of the alignment. Several test cases are used to demonstrate the efficiency of the tool. CONCLUSIONS Freely available NanoPipe software allows effortless and reliable analysis of MinION sequencing data for experienced bioinformaticians, as well for wet-lab biologists with minimum bioinformatics knowledge. Moreover, for the latter group, we describe the basic algorithm necessary for MinION sequencing analysis from the first to last step.

中文翻译：

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NanoPipe - 用于纳米孔 MinION 测序数据分析的网络服务器。

背景技术第三代长读长测序技术的快速进步将很快将生物和医学科学带入新的研究时代。总而言之，这项技术和实验程序变得更加简单，并且可供来自不同领域的生物学家使用，即使在 DNA 测序方面没有任何丰富的经验。因此，Oxford Nanopore Technologies 推出的 MinION 设备有望“将测序技术带给大众”，并允许在现场研究中进行快速、有效的分析。然而，这种测序技术的便利性与现有的分析工具形成鲜明对比，这可能会大大降低“普通”用户的热情。为了真正将测序技术带给每个生物学家，我们需要一套用户友好的工具，可以自动执行强大的分析。研究结果 NanoPipe 的开发考虑了 MinION 测序技术的具体情况，提供了相应调整的比对参数。用于比对的目标物种/序列的范围不受限制，NanoPipe 的描述性使用页面可帮助用户成功进行 NanoPipe 分析。结果包含比对统计、共有序列、多态性数据和比对可视化。使用几个测试用例来演示该工具的效率。结论 免费提供的 NanoPipe 软件可以让经验丰富的生物信息学家以及具有最少生物信息学知识的湿实验室生物学家轻松、可靠地分析 MinION 测序数据。此外，对于后一组，我们描述了 MinION 测序分析从第一步到最后一步所需的基本算法。