Objectives: Dentin hypersensitivity (DH) is a notable painful condition with a negative impact on quality of life. This paper evaluates the occluding abilities of nanohydroxyapatite synthesized from the eggshell waste (EnHAp) in the treatment of DH. Methods: EnHAp was synthesized following the wet chemical precipitation method. The purity and phase change were characterized using Fourier transform infrared (FTIR) and X-ray diffraction (XRD). Field emission scanning electron microscopy (FESEM) and high-resolution transmission electron microscopy (HRTEM) were used to observe the morphology of EnHAp. The occluding capabilities were evaluated in vitro using the bovine model. Twenty-five simulated dentin specimens were randomly assigned to five groups: Group 1, untreated; Group 2, eggshell powder alone; Group 3, calcined eggshell powder; Group 4, Colgate Sensitive treated, EnHAp; and Group 5, calcium dihydrogen phosphate (n = 5). The specimens were then subjected to remineralization protocol. The tubule occluding capabilities were observed using FESEM. Analysis of variance was used to analyze the occluding ratio values for all treated specimens. Post hoc test with Bonferroni correction was used to identify the mean differences among the five test groups (α = .05). Results: The FTIR and XRD results confirm that synthesis of EnHAp occurred. HRTEM and FESEM revealed an irregular rod structure with a particle size of 65 nm. The occluding area ratios observed for the EnHAp specimens were significantly higher than other test groups (p < 0.001). Conclusions: The application of EnHAp resulted in an efficient occlusion of dentin tubule. This study suggests that EnHAp is an effective therapy to treat DH.

中文翻译：

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从蛋壳废料中合成的纳米羟基磷灰石在封闭牙本质小管中的体外评价

目的：牙本质过敏（DH）是一种显着的疼痛状况，对生活质量有负面影响。本文评估了由蛋壳废料 (EnHAp) 合成的纳米羟基磷灰石在治疗 DH 中的封闭能力。方法：采用湿化学沉淀法合成EnHAp。使用傅里叶变换红外（FTIR）和X射线衍射（XRD）表征纯度和相变。场发射扫描电子显微镜（FESEM）和高分辨率透射电子显微镜（HRTEM）用于观察EnHAp的形态。使用牛模型在体外评估闭塞能力。将 25 个模拟牙本质样本随机分配到 5 组： 1 组，未处理；第2组，单独使用蛋壳粉；第3组，煅烧蛋壳粉；第 4 组，高露洁敏感处理，EnHAp；第 5 组，磷酸二氢钙 (n = 5)。然后对样本进行再矿化方案。使用 FESEM 观察小管闭塞能力。方差分析用于分析所有处理标本的咬合比值。使用 Bonferroni 校正的事后检验来确定五个测试组之间的平均差异 (α = .05)。结果：FTIR和XRD结果证实发生了EnHAp的合成。HRTEM 和 FESEM 揭示了粒径为 65 nm 的不规则棒状结构。观察到的 EnHAp 样本的遮挡面积比显着高于其他测试组 (p < 0.001)。结论：EnHAp 的应用导致牙本质小管的有效闭塞。