Background. Neuropathic pain not only affects individual life quality but also increases economic burden for the society. Treatment to alleviate neuropathic pain is required. Methodology. Fifty rats were randomly assigned into sham, spinal nerve ligation, and three treatment groups with different doses of Tempol (100, 200, and 300 mg/kg, respectively), with 10 rats in each group. A neuropathic pain model was created with spinal nerve L5 and L6 ligation. Mechanical allodynia and thermal hyperalgesia were tested preoperatively (day 0) and postoperatively (days 1, 3, 5, and 7). Spinal cord levels of nitric oxide, as well as activities of nitric oxide synthase and acetylcholinesterase, were tested in postoperative day 7. Results. Compared with rats in the spinal nerve ligation group, rats in Tempol treatment groups had decreased responses to mechanical pain and cold plate stimulations. A high dose of Tempol produced more attenuating effects. The level of nitric oxide and activity of nitric oxide synthase were also decreased with Tempol treatments, whereas no significant changes were observed in the activity of acetylcholinesterase. Conclusions. Tempol attenuated an experimental rat model with neuropathic pain by inhibiting nitric oxide production.

中文翻译：

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Tempol 通过抑制一氧化氮的产生来减轻神经性疼痛。

背景。神经性疼痛不仅影响个人生活质量，也增加了社会的经济负担。需要治疗以减轻神经性疼痛。方法论。将50只大鼠随机分为假手术组、脊神经结扎组和三个不同剂量Tempol（分别为100、200和300 mg/kg）的治疗组，每组10只大鼠。用脊神经 L5 和 L6 结扎创建神经性疼痛模型。机械异常性疼痛和热痛觉过敏在术前（第 0 天）和术后（第 1、3、5 和 7 天）进行了测试。在术后第 7 天检测脊髓的一氧化氮水平，以及一氧化氮合酶和乙酰胆碱酯酶的活性。结果. 与脊神经结扎组大鼠相比，Tempol 治疗组大鼠对机械疼痛和冷板刺激的反应降低。高剂量的 Tempol 产生更多的衰减效果。Tempol 处理的一氧化氮水平和一氧化氮合酶活性也降低，而乙酰胆碱酯酶活性未观察到显着变化。结论。Tempol 通过抑制一氧化氮的产生来减轻患有神经性疼痛的实验性大鼠模型。