Poly(L-lactide-co-ε-caprolactone) scaffolds were functionalised by 10 or 20 µg/mL of human demineralised dentine matrix. Release kinetics up to 21 days and their osteogenic potential on human bone marrow stromal cells after 7 and 21 days were studied. A total of 390 proteins were identified by mass spectrometry. Bone regeneration proteins showed initial burst of release. Human bone marrow stromal cells were cultured on scaffolds physisorbed with 20 µg/mL and cultured in basal medium (DDM group) or physisorbed and cultured in osteogenic medium or cultured on non-functionalised scaffolds in osteogenic medium. The human bone marrow stromal cells proliferated less in demineralised dentine matrix group and activated ERK/1/2 after both time points. Cells on DDM group showed highest expression of IL-6 and IL-8 at 7 days and expressed higher collagen type 1 alpha 2, SPP1 and bone morphogenetic protein-2 until 21 days. Extracellular protein revealed higher collagen type 1 and bone morphogenetic protein-2 at 21 days in demineralised dentine matrix group. Cells on DDM group showed signs of mineralisation. The functionalised scaffolds were able to stimulate osteogenic differentiation of human bone marrow stromal cells.

中文翻译：

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共聚物支架释放人类脱矿质牙本质基质进行骨再生的功效。

聚（L-丙交酯-ε-己内酯）支架通过10或20 µg / mL的人脱盐的牙本质基质进行功能化。研究了长达21天的释放动力学及其在7和21天后对人骨髓基质细胞的成骨潜力。通过质谱鉴定总共390种蛋白质。骨再生蛋白显示出最初的释放爆发。将人骨髓基质细胞培养在用20 µg / mL物理吸附的支架上，然后在基础培养基（DDM组）中培养，或者在成骨培养基中物理吸附和培养，或者在成骨培养基中在无功能的支架上培养。在两个时间点之后，去矿质的牙本质基质组中的人类骨髓基质细胞增殖较少，并激活了ERK / 1/2。DDM组的细胞在第7天显示最高的IL-6和IL-8表达，并在21天之前表达更高的1型胶原2α，SPP1和骨形态发生蛋白2。脱矿的牙本质基质组在21天时细胞外蛋白显示较高的1型胶原蛋白和2型骨形态发生蛋白。DDM组的细胞显示出矿化迹象。功能化的支架能够刺激人骨髓基质细胞的成骨分化。