Supplemental Digital Content is available in the text. Loss of immunoreactivity in tissue sections has been shown to occur when slide sections are stored at room temperature for prolonged periods of time. We conducted a systematic investigation to determine the extent of staining loss in various storage conditions to determine an optimal storage method. We investigated 6 antibodies that are commonly used for breast cancer subtyping in research studies with immunohistochemistry (ER, PR, HER2, CK5/6, EGFR, and Ki67) in formalin-fixed paraffin-embedded breast tissue microarrays consisting of 148 patients. Tissue microarrays were sectioned at various time points: fresh, 1 week, 1 month, 6 months, and 12 months before staining. Slides sectioned at each time point were stored in 5 storage conditions: desiccator, paraffin dipped, 4°C, −20°C, and −80°C. Immunohistochemistry scores were assessed over time with McNemar Test and Bowker Test of Symmetry. Desiccator storage was the only storage condition that did not show any loss in immunoreactivity for any antibody or time point in our study. Paraffin coated slides were the most difficult storage method operationally and also showed the most loss in immunoreactivity. Storing sections in a desiccator was the most effective method for minimizing immunoreactivity loss. Cold storage at 4°C is an intermediate option that is not as protective as a desiccator, but offers the advantage of being accessible to virtually all research labs.

中文翻译：

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组织微阵列切片中乳腺癌亚型标记的储存条件和免疫反应性

文本中提供了补充数字内容。当载玻片切片在室温下长时间保存时，组织切片会丧失免疫反应性。我们进行了系统调查，以确定各种储存条件下染色损失的程度，以确定最佳储存方法。我们在由 148 名患者组成的福尔马林固定石蜡包埋乳腺组织微阵列中使用免疫组织化学（ER、PR、HER2、CK5/6、EGFR 和 Ki67）研究了 6 种常用于乳腺癌亚型分型的抗体。在不同时间点对组织微阵列进行切片：新鲜、1 周、1 个月、6 个月和染色前 12 个月。在每个时间点切片的载玻片在 5 种储存条件下储存：干燥器、石蜡浸渍、4°C、-20°C 和 -80°C。使用 McNemar 检验和 Bowker 对称检验随时间评估免疫组织化学评分。在我们的研究中，干燥器储存是唯一没有显示出任何抗体或时间点的免疫反应性损失的储存条件。石蜡涂层载玻片是操作上最困难的存储方法，并且免疫反应性损失也最大。将切片储存在干燥器中是减少免疫反应性损失的最有效方法。4°C 冷藏是一种中间选择，它不像干燥器那样具有保护性，但具有几乎所有研究实验室都可以使用的优势。在我们的研究中，干燥器储存是唯一没有显示出任何抗体或时间点的免疫反应性损失的储存条件。石蜡涂层载玻片是操作上最困难的存储方法，并且免疫反应性损失也最大。将切片储存在干燥器中是减少免疫反应性损失的最有效方法。4°C 冷藏是一种中间选择，它不像干燥器那样具有保护性，但具有几乎所有研究实验室都可以使用的优势。在我们的研究中，干燥器储存是唯一没有显示出任何抗体或时间点的免疫反应性损失的储存条件。石蜡涂层载玻片是操作上最困难的存储方法，并且免疫反应性损失也最大。将切片储存在干燥器中是减少免疫反应性损失的最有效方法。4°C 冷藏是一种中间选择，它不像干燥器那样具有保护性，但具有几乎所有研究实验室都可以使用的优势。