Spinosad, a member of polyketide-derived macrolides produced in the actinomycete Saccharopolyspora spinosa, has been developed as a broad-spectrum and effective insecticide. The β-oxidation pathway could be an important source of building blocks for the biosynthesis of spinosad, thus the effect of vegetable oils on the production of spinosad in a high-yield strain was investigated. The spinosad production increased significantly with the addition of strawberry seed oil (511.64 mg/L) and camellia oil (520.07 mg/L) compared to the control group without oil (285.76 mg/L) and soybean oil group (398.11 mg/L). It also revealed that the addition of oils would affect the expression of genes involved in fatty acid metabolism, precursor supply, and oxidative stress. The genetically engineered strain, in which fadD1 and fadE genes of Streptomyces coelicolor were inserted, produced spinosad up to 784.72 mg/L in the medium containing camellia oil, while a higher spinosad production level (843.40 mg/L) was detected with the addition of 0.01 mM of thiourea.

中文翻译：

---

利用高产糖多孢菌棘孢菌株中的油利用和β-氧化操纵改善棘孢菌素的生产。

Spinosad是在放线菌Saccharopolyspora spinosa中产生的聚酮化合物衍生的大环内酯类的成员，已被开发为广谱有效的杀虫剂。β-氧化途径可能是多杀菌素生物合成的重要基础，因此，研究了植物油对高产菌株多杀菌素生产的影响。与不含油的对照组（285.76 mg / L）和大豆油组（398.11 mg / L）相比，添加草莓籽油（511.64 mg / L）和山茶油（520.07 mg / L）的菠菜产量显着增加。 。它还揭示了添加油会影响参与脂肪酸代谢，前体供应和氧化应激的基因的表达。基因工程菌株