Objetives: The goal of this study was to determine if systemic and peritoneal oxidative stress biomarkers are related to each other and to retrograde menstruation in endometriosis.

Methods: Plasma and peritoneal fluid oxidative stress biomarkers and hemoglobin and erythrocytes in peritoneal fluid as retrograde menstruation indicators, were measured in 28 patients with endometriosis and 23 without endometriosis.

Results: In the peritoneal fluid, carbonyls and lipohydroperoxides, indicative of protein and lipid oxidative damage, were higher in endometriosis group (21%, p = 0.016 and 46%, p = 0.009, respectively). However, these biomarkers were not different in the blood plasma of both groups, and only protein dityrosine, was increased in the plasma of endometriosis group (31%, p = 0.04). The peritoneal fluid hemoglobin content was not higher in the endometriosis group, nor related to carbonyls and lipohydroperoxides. Additionally, the peritoneal fluid oxidative biomarkers were not correlated with the blood plasma ones, and only malondialdehyde, and ischemia-modified albumin were almost two times higher in peritoneal fluid.

Discussion: Our results show a peritoneal and systemic oxidative stress biomarkers increase in endometriosis, but not related to each other, and do not support the hypothesis of an increase in hemoglobin-iron supply towards the peritoneal cavity that causes oxidative damage.

物体：这项研究的目的是确定体内和腹膜氧化应激生物标记物是否相互关联，以及子宫内膜异位症中的月经逆行。

方法：对28例子宫内膜异位症患者和23例非子宫内膜异位症患者进行血浆和腹膜液氧化应激生物标志物以及腹膜液中的血红蛋白和红细胞作为逆行月经指标。

结果：在子宫内膜异位症组中，腹膜液中的羰基和脂质过氧化氢较高，表明蛋白质和脂质的氧化损伤（分别为21％，p = 0.016和46％，p = 0.009）。然而，这些生物标志物在两组的血浆中没有差异，子宫内膜异位组的血浆中仅蛋白质二氢酪氨酸增加（31％，p = 0.04）。子宫内膜异位症组的腹膜液血红蛋白含量不高，也与羰基化合物和脂质过氧化氢无关。此外，腹膜液中的氧化生物标志物与血浆中的生物标志物不相关，仅丙二醛和缺血修饰的白蛋白在腹膜中的含量几乎高出两倍。

讨论：我们的结果显示，子宫内膜异位症的腹膜和全身氧化应激生物标志物增加，但彼此之间不相关，并且不支持针对腹膜腔的血红蛋白铁供应增加而引起氧化损伤的假说。