Alicyclobacillus acidoterrestris is considered to be one of the most important target microorganisms in quality control of heat-processed acidic foods. This species can cause spoilage by forming spores with very high heat resistance; however, no gas production or visible changes occur in the contaminated product during storage. Thus, the differentiation of A. acidoterrestris from other Alicyclobacillus species is of great importance. Τhe present study aims to find a rapid method for the identification of A. acidoterrestris. To achieve this, 78 Alicyclobacillus isolates were subjected to PCR-RFLP (restriction fragment length polymorphism). Specifically, PCR products of amplified V1-V3 region of the 16S rRNA gene were digested with restriction endonuclease HhaI. According to the obtained results, all A. acidoterrestris isolates showed similar restriction fragments of the 16S rRNA gene and different from A. acidocaldarius and A. hesperidum. In conclusion, a single enzyme PCR-RFLP assay was developed and showed rapid, inexpensive and direct identification of Alicyclobacillus isolates. The application of this method will be useful to identify this contaminant in fruit juices.

中文翻译：

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靶向16S rRNA基因V1-V3区域的单酶PCR-RFLP分析法，用于直接鉴定其他脂环酸杆菌的酸环脂环酸杆菌。

酸热脂环酸杆菌被认为是热加工酸性食品质量控制中最重要的目标微生物之一。这种物质会形成耐热性很高的孢子，从而导致腐败。但是，在存储过程中，受污染的产品不会产生气体或可见的变化。因此，将嗜酸曲霉与其他脂环芽孢杆菌种区分开是非常重要的。本研究旨在寻找一种鉴定A的快速方法。酸角藜。为了达到这个目标，需要78种脂环酸杆菌对分离物进行PCR-RFLP（限制性片段长度多态性）。具体而言，16S rRNA基因的扩增的V1-V3区的PCR产物进行消化，用限制性内切酶HHA I.根据获得的结果，所有A.耐热菌分离株表现出的16S rRNA基因的相似的限制性片段，并从不同A.热硫化叶菌和桔梗。总之，开发了一种单酶PCR-RFLP分析方法，该方法显示了快速，廉价和直接鉴定脂环酸杆菌分离株的方法。该方法的应用将有助于识别果汁中的这种污染物。