This study investigated the environmental factors associated with the presence of Vibrionaceae in economically important cage-cultured tropical marine fishes: the Asian Seabass Lates calcarifer, snapper Lutjanus sp., and hybrid grouper Epinephelus sp. Fish sampling was conducted at monthly intervals between December 2016 and August 2017. The body weight and length of individual fish were measured, and the skin, eye, liver, and kidney were sampled for bacterial isolation and identification. Water physicochemical parameters during the sampling activities were determined, and the enumeration of total Vibrionaceae count was also conducted from water and sediment samples. Nine species of Vibrio were identified, including V. alginolyticus, V. diabolicus, V. harveyi, V. campbellii, V. parahaemolyticus, V. rotiferianus, V. furnissii, V. fluvialis, and V. vulnificus. Photobacterium damselae subsp. damselae was also identified. A total of 73% of the isolated Vibrio belonged to the Harveyi clade, followed by the Vulnificus clade (5.5%) and Cholera clade (0.6%). Highest occurrence of Vibrio spp. and P. damselae subsp. damselae was found in hybrid grouper (72%), followed by Asian Seabass (48%) and snapper (36%). The associations of Vibrio spp. and P. damselae subsp. damselae with the host fish were not species specific. However, fish mortality and fish size showed strong associations with the presence of some Vibrio spp. On average, 60% of the infected cultured fish exhibited at least one clinical sign. Nevertheless, inconsistent associations were observed between the pathogens and water quality. The yearlong occurrence and abundance of Vibrionaceae in the environmental components indicate that they might serve as reservoirs of these pathogens.

中文翻译：

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与热带网箱养殖海水鱼类中弧菌科存在相关的环境因素

本研究调查了与具有重要经济意义的网箱养殖热带海洋鱼类中弧菌科的存在相关的环境因素：亚洲鲈鱼、长鳍鲷和杂交石斑鱼石斑鱼。2016 年 12 月至 2017 年 8 月期间，每隔一个月进行一次鱼类取样。测量个体鱼的体重和长度，并取样皮肤、眼睛、肝脏和肾脏进行细菌分离和鉴定。测定了采样活动期间的水理化参数，并对水样和沉积物样品进行了弧菌科总计数的计数。确定了九种弧菌，包括溶藻弧菌、恶魔弧菌、哈维弧菌、坎贝尔弧菌、副溶血弧菌、轮状弧菌、弗尼弧弧菌、河流弧菌和 V. 创伤。少女光杆菌亚种。damselae 也被确认。总共有 73% 的分离弧菌属于 Harveyi 进化枝，其次是 Vulnificus 进化枝 (5.5%) 和霍乱进化枝 (0.6%)。弧菌出现率最高。和 P. damselae subsp。在杂交石斑鱼中发现了 damselae (72%)，其次是亚洲鲈鱼 (48%) 和鲷鱼 (36%)。弧菌的关联。和 P. damselae subsp。damselae 与寄主鱼不是物种特异性的。然而，鱼类死亡率和鱼类大小与某些弧菌属的存在密切相关。平均而言，60% 的受感染养殖鱼表现出至少一种临床症状。然而，在病原体和水质之间观察到不一致的关联。