Plasma lipid and glucose levels are important parameters for evaluating the onset and development of metabolic and cardiovascular diseases. In clinical and experimental studies of humans or mice, fasting is often required before testing plasma lipid and glucose levels. The rabbit is a valuable animal model for cardiovascular disease research. However, whether fasting is necessary for measuring plasma lipid and glucose levels in rabbits remains unclear. In the current study, 12 healthy Japanese white rabbits (males weighing 2.5-3.0 kg) were randomly divided into a chow diet group (n = 6) and a high cholesterol diet group (n = 6). They were fed either a standard chow diet or a chow diet supplemented with 0.5% cholesterol and 3% corn oil for 12 weeks. After 12 weeks, the plasma levels of total cholesterol, triglycerides, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol and glucose were measured before and after various fasting durations (8, 12, 16, 20 and 24 h). The results showed that there were no significant differences in lipid levels between the fasting and non-fasting samples, whereas glucose levels were lower after 8 h of fasting than in the absence of fasting. Moreover, the glucose levels were restored to normal after 8 h of refeeding. These results indicate that fasting does not affect plasma lipid values in rabbits but that fasting is important for determining the glucose level in rabbits. These findings may be helpful for future rabbit experiments and beneficial for animal welfare.

中文翻译：

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测量兔子血浆脂质水平不需要空腹。

血浆脂质和葡萄糖水平是评估代谢和心血管疾病的发作和发展的重要参数。在人类或小鼠的临床和实验研究中，通常需要在测试血浆脂质和葡萄糖水平之前禁食。兔子是用于心血管疾病研究的有价值的动物模型。但是，尚不清楚是否需要禁食来测量兔的血浆脂质和葡萄糖水平。在本研究中，将12只健康的日本白兔子（雄性体重在2.5-3.0千克之间）随机分为成年饮食组（n = 6）和高胆固醇饮食组（n = 6）。他们接受标准的日常饮食或补充有0.5％胆固醇和3％玉米油的日常饮食12周。12周后，血浆总胆固醇，甘油三酸酯，在各种禁食持续时间（8、12、16、20和24小时）之前和之后测量高密度脂蛋白胆固醇，低密度脂蛋白胆固醇和葡萄糖。结果显示，空腹和非空腹样品之间的脂质水平没有显着差异，而空腹8h后的葡萄糖水平比没有空腹的情况低。此外，再喂食8小时后，葡萄糖水平恢复正常。这些结果表明，禁食不影响兔子的血浆脂质值，但是禁食对于确定兔子的葡萄糖水平很重要。这些发现可能对将来的兔子实验有帮助，也对动物福利有益。结果显示，空腹和非空腹样品之间的脂质水平没有显着差异，而空腹8h后的葡萄糖水平比没有空腹的情况低。此外，再喂食8小时后，葡萄糖水平恢复正常。这些结果表明，禁食不影响兔子的血浆脂质值，但是禁食对于确定兔子的葡萄糖水平很重要。这些发现可能对将来的兔子实验有帮助，也对动物福利有益。结果显示，空腹和非空腹样品之间的脂质水平没有显着差异，而空腹8h后的葡萄糖水平比没有空腹的情况低。此外，再喂食8小时后，葡萄糖水平恢复正常。这些结果表明，禁食不影响兔子的血浆脂质值，但是禁食对于确定兔子的葡萄糖水平很重要。这些发现可能对将来的兔子实验有帮助，也对动物福利有益。这些结果表明，禁食不影响兔子的血浆脂质值，但是禁食对于确定兔子的葡萄糖水平很重要。这些发现可能对将来的兔子实验有帮助，也对动物福利有益。这些结果表明，禁食不影响兔子的血浆脂质值，但是禁食对于确定兔子的葡萄糖水平很重要。这些发现可能对将来的兔子实验有帮助，也对动物福利有益。