Color Index (C.I.) Disperse Blue 291 (DB291) is an azo dye used by the textile industry. After yarn dyeing, wastewater containing the dye, released into the aquatic environment, may pollute drinking water sources. We investigated the mutagenicity and genotoxicity of DB291 in male Swiss mice, following oral administration. Micronucleated cells, primary DNA damage (comet assay) in blood, liver, and kidney cells, and BAX, BCL2, SMAD4 and TNFA gene expression in leukocytes were evaluated. An increased frequency of micronucleated polychromatic erythrocytes (MNPCEs) was observed in animals treated with 50 mg/kg bw; no other genetic alteration was detected. Neither primary DNA damage nor changes in gene expression were observed.

中文翻译：

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纺织染料CI分散蓝291对小鼠骨髓的遗传毒性。

色指数（CI）分散蓝291（DB291）是纺织工业使用的偶氮染料。纱线染色后，含有染料的废水释放到水生环境中可能会污染饮用水源。口服给药后，我们调查了DB291在雄性瑞士小鼠中的致突变性和遗传毒性。评估了微核细胞，血液，肝和肾细胞中的原发性DNA损伤（彗星测定）以及白细胞中的BAX，BCL2，SMAD4和TNFA基因表达。在用50 mg / kg bw处理的动物中，观察到微核多色红细胞（MNPCE）的频率增加；未检测到其他遗传改变。没有观察到原发性DNA损伤或基因表达的变化。