PEAR1 is highly expressed at bovine MDSC differentiation. However, its biological function remains unclear. Western blotting results showed that PEAR1 increased between day 0 and day 2 of cell differentiation and decreased from day 3. Moreover, scratch test showed that wound healing rate increased after PEAR1 overexpression and decreased upon its suppression. Meanwhile, we found that, upon PEAR1 induction, both the expression of the focal adhesion-associated and MyoG, and the myotube fusion rate increased. However, when PEAR1 was suppressed, opposite results were obtained. Immunoprecipitation revealed an association between PEAR1 and ITGB1. Notably, inhibition of FAK and ITGB1 repressed cell differentiation. In conclusion, our study indicated that PEAR1 is involved in the regulation of bovine MDSC migration and differentiation.

中文翻译：

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血小板内皮聚集受体1通过整联蛋白β-1和粘着斑激酶调节牛肌肉卫星细胞的迁移和分化。

PEAR1在牛MDSC分化中高度表达。但是，其生物学功能仍不清楚。Western印迹结果表明，PEAR1在细胞分化的第0天和第2天之间增加，而从第3天开始下降。此外，刮擦试验表明，PEAR1过表达后伤口愈合速度增加，而其抑制后伤口愈合速度下降。同时，我们发现，在PEAR1诱导后，粘着斑相关的表达和MyoG均增加，并且肌管融合率均增加。但是，当PEAR1被抑制时，获得相反的结果。免疫沉淀显示PEAR1和ITGB1之间存在关联。值得注意的是，抑制FAK和ITGB1可抑制细胞分化。总之，我们的研究表明PEAR1参与了牛MDSC迁移和分化的调控。