β‐1,4‐Galactanases are glycoside hydrolases that are involved in the degradation of pectin and belong to family 53 in the classification of glycoside hydrolases. Previous studies have elucidated the structures of several fungal and two bacterial galactanases, while biochemical studies have indicated differences in the product profiles of different members of the family. Structural studies of ligand complexes have to date been limited to the bacterial members of the family. Here, the first structure of a fungal galactanase in complex with a disaccharide is presented. Galactobiose binds to subsites −1 and −2, thus improving our understanding of ligand binding to galactanases.

中文翻译：

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刺曲霉β-1,4-半乳聚糖酶与半乳糖二糖复合的结构。

β-1,4-半乳糖苷酶是糖苷水解酶，参与果胶的降解，属于糖苷水解酶分类中的53族。先前的研究已经阐明了几种真菌和两种细菌半乳聚糖酶的结构，而生化研究表明该家族不同成员的产品特征存在差异。迄今为止，配体复合物的结构研究仅限于该家族的细菌成员。在此，提出了一种真菌半乳聚糖酶与二糖复合的第一结构。半乳糖二糖与亚位点-1和-2结合，从而提高了我们对配体与半乳糖酶结合的理解。