BACKGROUND Polycystic ovary syndrome (PCOS) is a complex disorder associated with multiple metabolic disturbance, including defective glucose metabolism and insulin resistance. The altered metabolites caused by the related metabolic disturbance may affect ovarian follicles, which can be reflected in follicular fluid composition. The aim of this study is to investigate follicular fluid metabolic profiles in women with PCOS using an advanced sequential window acquisition of all theoretical fragment-ion spectra (SWATH) mass spectrometry. MATERIALS AND METHODS Nineteen women with PCOS and twenty-one healthy controls undergoing IVF/ET were recruited, and their follicular fluid samples were collected for metabolomic study. Follicular fluid metabolic profiles, including steroid hormones, free fatty acids, bioactive lipids, and amino acids were analyzed using the principal component analysis (PCA) and partial least squares to latent structure-discriminant analysis (PLS-DA) model. RESULTS Levels of free fatty acids, 3-hydroxynonanoyl carnitine and eicosapentaenoic acid were significantly increased (P < 0.05), whereas those of bioactive lipids, lysophosphatidylcholines (LysoPC) (16:0), phytosphingosine, LysoPC (14:0) and LysoPC (18:0) were significantly decreased in women with PCOS (P < 0.05). Additionally, levels of steroid hormone deoxycorticosterone and two amino acids, phenylalanine and leucine were higher in the PCOS patients (P < 0.05). CONCLUSION Women with PCOS display unique metabolic profiles in their follicular fluid, and this data may provide us with important biochemical information and metabolic signatures that enable a better understanding of the pathogenesis of PCOS.

中文翻译：

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通过SWATH质谱鉴定卵泡液中多囊卵巢综合征的潜在代谢生物标志物。

背景技术多囊卵巢综合征（PCOS）是与多种代谢紊乱相关的复杂疾病，包括葡萄糖代谢缺陷和胰岛素抵抗。由相关的代谢紊乱引起的代谢物改变可能影响卵巢卵泡，这可以反映在卵泡液成分中。这项研究的目的是使用所有理论碎片离子光谱（SWATH）质谱的高级连续窗口采集方法来研究PCOS妇女的卵泡液代谢谱。材料与方法招募了19名PCOS妇女和21名接受IVF / ET的健康对照，并收集了她们的卵泡液样本用于代谢组学研究。卵泡液代谢概况，包括类固醇激素，游离脂肪酸，生物活性脂质，氨基酸使用主成分分析（PCA）和偏最小二乘法进行潜在结构鉴别分析（PLS-DA）模型进行分析。结果游离脂肪酸，3-羟基壬酰基肉碱和二十碳五烯酸的水平显着升高（P <0.05），而生物活性脂质，溶血磷脂酰胆碱（LysoPC）（16：0），植物鞘氨醇，LysoPC（14：0）和LysoPC（患有PCOS的女性中18：0）显着降低（P <0.05）。此外，PCOS患者的类固醇激素脱氧皮质酮和两种氨基酸（苯丙氨酸和亮氨酸）水平较高（P <0.05）。结论患有PCOS的女性在其卵泡液中显示出独特的代谢特征，