Diamides have been used worldwide to manage the diamondback moth (DBM),Plutella xylostellaL. (Lepidoptera: Plutellidae), however some strains showed resistance to these molecules. Also, pheromone traps could be used to manage this pest, hence reducing the use of insecticides in the field. Resistant DBM strains may have biological disadvantages in comparison to susceptible strains in areas without sprays, including reduction in fitness or behavioral changes. Therefore, the aim of this study was to investigate whether DBM strains resistant to chlorantraniliprole showed adaptive costs that could alter male attraction to the sex pheromone, in comparison to susceptible strains in the laboratory and semi-field conditions. First, the LC1, LC10, LC25, and LC50of DBM to chlorantraniliprole were established, which were 0.003, 0.005, 0.007, and 0.011 mg a.i. liter−1, and 5.88, 24.80, 57.22, and 144.87 mg a.i. liter−1for the susceptible and resistant strains, respectively. Development and reproduction of DBM strains subjected to those concentrations were compared. Later, male response to the sex pheromone was investigated in a Y-tube in the laboratory and in a greenhouse to pheromone traps. Resistant DBM strain showed an adaptive cost in comparison to the susceptible strain that can result in a delay in population growth in the field when selection pressure is absent. Conversely, resistant males have no olfactory response alteration in comparison to susceptible males, consistently at 3 (P= 0.6848) and 7 days (P= 0.9140) after release, suggesting that pheromone traps continue to be a viable alternative to manage DBM in an IPM system.

中文翻译：

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抗性和易感小菜蛾氯虫苯甲酰胺的行为反应和适应成本

二酰胺已在世界范围内用于管理小菜蛾 (DBM)，小菜蛾L.（鳞翅目：Plutellidae），然而一些菌株对这些分子表现出抗性。此外，信息素诱捕器可用于控制这种害虫，从而减少田间杀虫剂的使用。与没有喷雾的区域中的敏感菌株相比，耐药 DBM 菌株可能具有生物学劣势，包括适应性降低或行为改变。因此，本研究的目的是调查与实验室和半田间条件下的易感菌株相比，对氯虫苯甲酰胺具有抗性的 DBM 菌株是否表现出可能改变男性对性信息素的吸引力的适应性成本。一、LC1, 液相色谱10, 液相色谱25, 和 LC50DBM 与氯虫苯甲酰胺的比值分别为 0.003、0.005、0.007 和 0.011 mg ai L-1和 5.88、24.80、57.22 和 144.87 毫克铝升-1分别为易感菌株和耐药菌株。比较了经受这些浓度的 DBM 菌株的发育和繁殖。后来，男性对性信息素的反应在实验室的 Y 管和温室中对信息素陷阱进行了调查。与易感菌株相比，抗性 DBM 菌株显示出适应性成本，当没有选择压力时，这可能导致田间种群增长的延迟。相反，与易感雄性相比，耐药雄性没有嗅觉反应改变，始终为 3 (磷= 0.6848) 和 7 天 (磷= 0.9140），表明信息素陷阱仍然是在 IPM 系统中管理 DBM 的可行替代方案。