The central point of current investigations was the first time ever synthesis of modern substitutes of human milk fat followed by versatile evaluation of their oxidative properties. The enzymatic interesterification conducted at 70°C for 2, 4, and 6 hours, respectively, with milk thistle oil and lard blend as starting reactants was catalyzed by 1,3-specific lipase Lipozyme RM IM, obtained from Rhizomucor miehei. Pressure Differential Scanning Calorimetry (PDSC) and Fourier Transform Infrared Spectroscopy (FT-IR) were applied to evaluate quality of products formed. Although PDSC curves showed lower oxidative stability of newly synthesized fats as compared to both starting materials separately, they can be considered adequate substitutes of human fat milk fat, as distribution of fatty acids in triacylglycerol molecules of substitutes obtained is much alike human milk fat itself, as resulted from analysis of GC data collected. Obvious changes in chemical structure of fats occurring during interesterification resulted in specific alterations in IR spectra of processed materials. Spectral data accompanied by PLS technique were successfully used for accurate determination of oxidative stability of new fats through indirect procedure, i.e., IR-PDSC-reference analysis of induction time. Additionally IR data exclusively, i.e., without any reference data, occurred powerfully in discrimination of human fat milk substitutes obtained.

中文翻译：

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合成后，通过光谱和量热评估从蓟牛奶和猪油中获得的人乳脂肪替代物的稳定性。

当前研究的重点是首次合成人乳脂肪的现代替代品，随后对其氧化性质进行了综合评估。以从蓟草油和猪油混合物作为起始反应物在70℃分别进行2、4和6小时的酶促酯交换反应，该酶由获自Rhizomucor miehei的1,3-特异性脂肪酶Lipozyme RM IM催化。压差扫描量热法（PDSC）和傅立叶变换红外光谱（FT-IR）用于评估所形成产品的质量。尽管PDSC曲线分别显示出与两种起始原料相比，新合成脂肪的氧化稳定性较低，但可以认为它们是人脂肪，乳脂的适当替代品，因为获得的替代品的三酰基甘油分子中的脂肪酸分布与人乳脂本身非常相似，这是根据收集的GC数据分析得出的。酯交换过程中发生的脂肪化学结构的明显变化导致加工材料的红外光谱发生特定变化。通过间接程序，即诱导时间的IR-PDSC参考分析，成功地将结合PLS技术的光谱数据用于准确测定新脂肪的氧化稳定性。另外，IR数据排他性地（即没有任何参考数据）在辨别所获得的人类脂肪乳替代品中有力地发生了。酯交换过程中发生的脂肪化学结构的明显变化导致加工材料的红外光谱发生特定变化。通过间接程序，即诱导时间的IR-PDSC参考分析，成功地将结合PLS技术的光谱数据用于准确测定新脂肪的氧化稳定性。另外，IR数据排他性地（即没有任何参考数据）在辨别所获得的人类脂肪乳替代品中有力地发生了。酯交换过程中发生的脂肪化学结构的明显变化导致加工材料的红外光谱发生特定变化。通过间接程序，即诱导时间的IR-PDSC参考分析，成功地将结合PLS技术的光谱数据用于准确测定新脂肪的氧化稳定性。另外，IR数据排他性地（即没有任何参考数据）在辨别所获得的人类脂肪乳替代品中有力地发生了。