Iodinated radiographic contrast media is used in cancer radiography for cancer diagnosis. The aim of this present study was to examine five iodinated radiographic contrast media (IRCM) (i.e., iohexol, iopamidol, iobitridol, ioxaglate, and iodixanol) in terms of their cytotoxicity, mitochondria membrane potential (ΔΨm), and P-glycoprotein function in multidrug resistant K562/Dox cancer cells and corresponding sensitive cancer cells. The cytotoxicity was determined by colorimetric resazurin reduction assay. The ΔΨm and P-glycoprotein function was measured using a noninvasive functional spectrofluorometry. Rhodamine B, fluorescence probe, was used to estimate ΔΨm. The kinetic of P-glycoprotein-mediated efflux pirarubicin was used to monitor P-glycoprotein function in multidrug resistant (MDR) cancer cells. The results showed that ioxaglate and iodixanol show similar efficacy in MDR cancer cells and for their corresponding sensitive cancer cells. Iopamidol, iohexol, and iobitridol showed higher efficacy in MDR cancer cells than for the corresponding sensitive cancer cells by approximately 2 fold. The results also showed no significant change in the |ΔΨm| values in treated K562 and K562/Dox cancer cells when compared to the non-treated K562 and K562/Dox cancer cells. However, there were notable changes detected for iobitridol and iodixanol at 50 mgI/mL. Similarly, the results showed significant differences in P-glycoprotein function of K562/Dox cancer cells after treatment with IRCM when compared to the non-treated K562/Dox cancer cells, with iohexol and iodixanol being the notable exceptions once again. In this present study, IRCM exhibited cytotoxicity on MDR cancer cells and their corresponding sensitive cancer cells. IRCM also showed potential as an anticancer agent in the future.

中文翻译：

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碘化射线造影剂对耐多药的K562 / Dox细胞的影响：线粒体受损和P-糖蛋白抑制。

碘射线照相造影剂用于癌症射线照相以诊断癌症。本研究的目的是从细胞毒性，线粒体膜电位（ΔΨm）和P-糖蛋白功能方面研究五种碘化造影剂（IRCM）（即碘海醇，碘帕醇，碘比妥醇，碘克沙酯和碘克沙醇）。多药耐药性K562 / Dox癌细胞和相应的敏感癌细胞。细胞毒性通过比色刃天青素还原测定法测定。使用无创功能性荧光分光光度法测量ΔΨm和P-糖蛋白功能。罗丹明B，荧光探针，用于估计ΔΨm。P糖蛋白介导的吡柔比星的动力学用于监测多药耐药（MDR）癌细胞中P糖蛋白的功能。结果表明，ioxaglate和碘克沙醇在MDR癌细胞及其相应的敏感癌细胞中显示出相似的功效。碘帕醇，碘海醇和碘比地洛在MDR癌细胞中的疗效比对相应的敏感性癌细胞高约2倍。结果还显示|ΔΨm|没有明显变化。与未处理的K562和K562 / Dox癌细胞相比，经处理的K562和K562 / Dox癌细胞的Tn值较高。但是，在50 mgI / mL的浓度下，检测到的iobitridol和iodixanol发生了显着变化。类似地，结果显示，与未处理的K562 / Dox癌细胞相比，IRCM处理后的K562 / Dox癌细胞的P-糖蛋白功能有显着差异，碘海醇和碘克沙醇再次成为显着例外。在本研究中，IRCM对MDR癌细胞及其相应的敏感癌细胞具有细胞毒性。IRCM在将来也显示出作为抗癌药的潜力。