It has been demonstrated that short peptides play an important role in the transmission of biological information, modulation of transcription, and restoring genetically conditioned alterations occurring with age. Peptidergic regulation of homeostasis occupies an important place in physiological processes, which lead to the aging of cells, tissues, and organs, consisting in the involution of major regulatory systems-the nervous, the endocrine, and the immune. The effect of AED (Ala-Glu-Asp), KED (Lys-Glu-Asp), KE (Lys-Glu), AEDG (Ala-Glu-Asp-Gly) peptides and their compound on neuronal differentiation of human periodontal ligament stem cells (hPDLSCs) was studied by immunofluorescence and western blot analysis. Growth-Associated Protein 43 (GAP43), which implements neurotransmission mechanisms and neuroplasticity, demonstrated an increased expression in hPDLSCs cultured with a compound of all studied peptides and with KED alone. The peptide compound and KED, increase the expression of Nestin (neurofilament protein), expressed in early neuronal precursors in hPDLSCs cultures. Thus, the compound of peptides AEDG, KE, AED, and KED could promote the neuronal differentiation of hPDLSCs and be a promising tool for the study of peptides as a modulator of neurogenesis in neurodegenerative diseases studied in animal models.

中文翻译：

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短肽对干细胞神经元分化的影响。

已经证明，短肽在生物信息的传递，转录的调节以及恢复随着年龄而发生的遗传条件改变中起重要作用。稳态的肽能调节在生理过程中占有重要地位，这导致细胞，组织和器官的衰老，包括主要调节系统（神经，内分泌和免疫）的参与。AED（Ala-Glu-Asp），KED（Lys-Glu-Asp），KE（Lys-Glu），AEDG（Ala-Glu-Asp-Gly）肽及其化合物对人牙周膜干神经元分化的影响通过免疫荧光和蛋白质印迹分析研究了细胞（hPDLSCs）。增长相关蛋白43（GAP43），它实现神经传递机制和神经可塑性，证明在用所有研究的肽的化合物和单独的KED培养的hPDLSC中表达增加。肽化合物和KED增加hPDLSCs培养物中早期神经元前体中表达的Nestin（神经丝蛋白）的表达。因此，肽AEDG，KE，AED和KED的化合物可以促进hPDLSCs的神经元分化，并成为研究肽作为动物模型中神经退行性疾病中神经发生调节剂的有前途的工具。