Capillary zone electrophoresis (CZE) paired with mass spectrometry (MS) is a powerful analytical technique for examining mixtures of ionic analytes such as glycosaminoglycans. This study examines the mechanics of the electrospray process for a sheath flow CZE-MS interface under reverse polarity negative ionization conditions. Liquid flow in a sheath flow nano-electrospray CZE-MS interface is driven by two mechanisms, electroosmotic flow and electrospray nebulization. The contribution of these two processes to the overall flow of solution to the electrospray tip is influenced by the surface coatings of the sheath flow emitter tip and by the solvent composition. We have investigated the application of this interface to the reverse polarity separation of glycosaminoglycans and find that the role of electroosmotic flow is far less than has been reported previously, and the electrospray process itself is the largest contributor to the flow of the sheath liquid.

中文翻译：

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反极性和负离子模式下毛细管电泳-质谱接口的电喷雾研究

毛细管区带电泳 (CZE) 与质谱 (MS) 相结合是一种强大的分析技术，用于检查离子分析物（如糖胺聚糖）的混合物。本研究检查了在反极性负电离条件下鞘流 CZE-MS 界面的电喷雾过程的力学原理。鞘流纳米电喷雾 CZE-MS 接口中的液体流动由两种机制驱动，即电渗流和电喷雾雾化。这两个过程对溶液到电喷雾尖端的整体流动的贡献受鞘流发射器尖端的表面涂层和溶剂组成的影响。