Regardless of the efforts put into preventing or reducing fungal growth, extensive mycotoxin contamination has been reported in animal feeds. In the case of pigs, one of the mycotoxins of major concern is deoxynivalenol (DON). The use of adsorbents as feed additives represents one of the strategies to control mycotoxins’ contamination in feedstuff. Therefore, the aim of the study was to verify the ability of chlorophyllin (CHL) to reduce the absorption rate of DON in swine mucosa explants. Intestine was obtained from routinely slaughtered adult pigs. The mucosa explants were studied by means of Ussing chamber technique. The effect of DON (10 and 30 μg/ml) on mucosa viability and permeability and CHL (100 μg/ml) impact on DON (30 μg/ml) absorption was verified. The results revealed that mucosa explants isolated from adult animals remained unaffected for 90 min in the presence of DON in the lower concentration (10 μg/ml). Mycotoxin in the higher dose (30 μg/ml) increased mucosa permeability (decreased transepithelial electrical resistance value) and enhanced paracellular transport of lucifer yellow and mannitol but did not affect lactate dehydrogenase leakage. The introduction of CHL neither diminished the absorption rate of DON across swine mucosa explants nor prevented the toxic effects of DON on intestine. In conclusion, the results confirm the negative effect of DON on pig jejunum mucosa. However, the toxic effect of DON was observed only when it was used in relatively high doses. A promising adsorbent agent, CHL, failed to reduce the intensity of DON transport across intestine under in vitro conditions.

中文翻译：

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叶绿素对脱氧雪腐镰刀菌烯醇跨空肠粘膜外植体转运的影响从成年猪获得。

尽管在预防或减少真菌生长方面付出了努力，但动物饲料中仍存在大量霉菌毒素污染的报道。就猪而言，主要关注的霉菌毒素之一是脱氧雪腐镰刀菌烯醇 (DON)。使用吸附剂作为饲料添加剂是控制饲料中霉菌毒素污染的策略之一。因此，本研究的目的是验证叶绿素（CHL）降低猪粘膜外植体中 DON 吸收率的能力。肠道取自常规屠宰的成年猪。通过尤斯室技术研究粘膜外植体。验证了 DON（10 和 30 μg/ml）对粘膜活力和渗透性的影响以及 CHL（100 μg/ml）对 DON（30 μg/ml）吸收的影响。结果显示，在较低浓度（10 μg/ml）的 DON 存在下，从成年动物分离的粘膜外植体在 90 分钟内保持不受影响。较高剂量（30 μg/ml）的霉菌毒素增加了粘膜通透性（降低了跨上皮电阻值）并增强了荧光黄和甘露醇的细胞旁转运，但不影响乳酸脱氢酶渗漏。CHL的引入既不会降低猪粘膜外植体对DON的吸收率，也不会阻止DON对肠道的毒性作用。总之，结果证实了 DON 对猪空肠粘膜的负面影响。然而，只有在相对高剂量使用时，才会观察到 DON 的毒性作用。一种有前途的吸附剂 CHL 在体外条件下未能降低 DON 穿过肠道的转运强度。