Vitamin B deficiency in patients with inflammatory bowel disease (IBD) is well-documented; however, few studies have explored genomic damage in patients with IBD using the cytokinesis-block micronucleus cytome (CBMN-Cyt) assay. This study investigated the frequency of micronuclei (MNi) using the CBMN-Cyt assay and the level of vitamin B in patients with IBD. This prospective study was conducted in 15 patients with ulcerative colitis, 15 patients with Crohn's disease, and 30 healthy controls from one tertiary hospital. Serum vitamin B and homocysteine levels were measured, and the MNi status was analyzed using the CBMN-Cyt assay. The patients with IBD showed significantly lower serum pyridoxine levels and significantly higher homocysteine levels than controls. The frequencies of binucleated cells (BNCs) with MNi, nucleoplasmic bridges (NPBs), and nuclear buds (Nbuds) were 8.5 [5.8-13.5], 1.0 [0.0-1.9], and 5.4 [4.3-7.4] for the IBD group, and 5.9 [4.8-7.7], 0.2 [0.0-1.0], and 3.5 [2.9-5.4] for the control group (P =  0.011, P =  0.010, and P =  0.002), respectively. This study suggests that patients with IBD have increased frequencies of MNi and decreased levels of pyridoxine than healthy controls.

中文翻译：

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炎性肠病患者的基因组损伤和维生素B状态增加：一项病例对照，前瞻性，先导研究。

炎症性肠病（IBD）患者中的维生素B缺乏症有据可查；然而，很少有研究使用胞质分裂阻滞性微核细胞因子（CBMN-Cyt）测定法探讨IBD患者的基因组损伤。这项研究使用CBMN-Cyt分析法研究了IBD患者的微核（MNi）频率和维生素B水平。这项前瞻性研究是在一家三级医院对15例溃疡性结肠炎，15例克罗恩病患者和30名健康对照进行的。测量血清维生素B和高半胱氨酸水平，并使用CBMN-Cyt分析法分析MNi状态。IBD患者的血清吡patients醇水平明显降低，同型半胱氨酸水平明显高于对照组。带有MNi，核质桥（NPB）的双核细胞（BNC）的频率，和IBD组的核芽（Nbuds）分别为8.5 [5.8-13.5]，1.0 [0.0-1.9]和5.4 [4.3-7.4]，以及5.9 [4.8-7.7]，0.2 [0.0-1.0]和3.5对照组的[2.9-5.4]（P = 0.011，P = 0.010和P = 0.002）。这项研究表明，与健康对照组相比，IBD患者的MNi发生频率增加，吡ido醇水平降低。