Exposure to ionizing radiation is unavoidable for noncancerous cells during the external radiotherapy process. Increasing the dose delivery fraction times leads to increasing the endothelial cell damage. Vascular abnormalities are commonly associated with the alternation of endothelium biomechanical properties. The goal of the present study was to quantify the elastic and viscoelastic properties of human umbilical vein endothelial cells (HUVECs) using the micropipette aspiration technique in conjunction with a theoretical model while an 8 Gy dose was given in four fractions. Confocal imaging was performed for evaluation of cytoskeletal changes during fractionation ⁶⁰Co radiotherapy. The results indicated an increase in elastic modulus from 29.87 ± 1.04 Pa to 46.69 ± 1.17 Pa while the fractional doses increased from 0 Gy to 8 Gy along with the obvious cytoskeletal changes. Moreover, in the creep behavior of radiated groups, a significant decrease was shown in the time constant and viscoelastic properties. On the other hand, it was observed that the change in the biomechanical properties of the cells while applying a single fraction of 8 Gy was not exactly the same as that in the properties of the radiation-exposed cells while delivering an 8 Gy dose at 2 Gy per fraction. The observed differences in the biomechanical behavior of endothelium provide a quantitative description of radiobiological effects for evaluating the dose-response relationship as a biological dosimetry procedure.

中文翻译：

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剂量依赖性60Coγ辐射对人内皮细胞力学性能的影响。

在外部放疗过程中，非癌细胞不可避免地要暴露于电离辐射中。增加剂量递送分数时间导致内皮细胞损伤增加。血管异常通常与内皮生物力学特性的改变有关。本研究的目的是使用微量移液器抽吸技术结合理论模型对人脐静脉内皮细胞（HUVEC）的弹性和粘弹性进行定量，同时将8 Gy剂量分四部分进行。进行共聚焦成像以评估分级分离过程中的细胞骨架变化⁶⁰联合放疗。结果表明，弹性模量从29.87±1.04 Pa增加到46.69±1.17 Pa，而部分剂量从0 Gy增加到8 Gy，同时细胞骨架发生明显变化。此外，在辐射基团的蠕变行为中，时间常数和粘弹性特性显着降低。另一方面，观察到在单次施用8 Gy时细胞的生物力学性能变化与在2 g下递送8 Gy剂量的放射线细胞的性能变化并不完全相同。每分数Gy。观察到的内皮生物力学行为的差异提供了放射生物学效应的定量描述，用于评估作为生物剂量测定程序的剂量反应关系。