Esterase 6 in Drosophila melanogaster, Drosophila simulans and Drosophila mauritiana is produced in several life stages and diverse tissues, but the major pulse of expression is in the sperm ejaculatory duct of adult males. Comparison of EST6 activity levels among several lines of D. melanogaster, D. simulans and D. mauritiana reveals two major quantitative differences among the species. First, newly eclosed females of both D. simulans and D. mauritiana show significantly higher EST6 activity than those of D. melanogaster. Secondly, 5-day-old adult D. simulans have significantly higher activities than D. mauritiana in both sexes and significantly higher activity than D. melanogaster in males. The genetic bases of the differences between D. melanogaster and the other species are investigated through germ line transfer of the D. simulans and D. mauritiana Est-6 genes plus 1.2 kb of 5′ and 0.2 kb of their 3′ flanking sequences into D. melanogaster. The newly eclosed female activities of the transformants resemble those of the two donor species, suggesting that the interspecific differences in this aspect of expression are due to cis-inherited factors contained within the transferred DNA. In contrast, the 5-day adult activity of the D. simulans transgene resembles the recipient species, D. melanogaster, suggesting that the difference between D. simulans and D. melanogaster in this aspect of expression is due to trans-acting factors. We also find that third instar larval activities of the D. simulans transgene and 5-day male activities of the D. mauritiana transgene are lower than those of either parental species, suggesting that not all the promoter elements relevant to these aspects of expression are included in the transferred DNA.

中文翻译：

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同胞果蝇物种中酯酶 6 活性变化的调节方面

Drosophila melanogaster、Drosophila simulans 和 Drosophila mauritiana 中的酯酶 6 在几个生命阶段和不同的组织中产生，但表达的主要脉冲是在成年雄性的射精管中。D. melanogaster、D. simulans 和 D. mauritiana 几个品系之间 EST6 活性水平的比较揭示了物种之间的两个主要数量差异。首先，D. simulans 和 D. mauritiana 的新封闭雌性显示出明显高于 D. melanogaster 的 EST6 活性。其次，5 日龄成年 D. simulans 的活性显着高于 D. mauritiana，雄性显着高于 D. melanogaster。通过模拟黑腹果蝇和黑腹果蝇的种系转移研究黑腹果蝇与其他物种之间差异的遗传基础。mauritiana Est-6 基因加上 1.2 kb 的 5' 和 0.2 kb 的 3' 侧翼序列进入黑腹果蝇。转化体新封闭的雌性活动类似于两个供体物种的活动，表明这方面的种间差异是由于转移的 DNA 中包含的顺式遗传因子。相比之下，D. simulans 转基因的 5 天成虫活性类似于受体物种 D. melanogaster，这表明 D. simulans 和 D. melanogaster 在这方面的表达差异是由于反式作用因素。我们还发现 D. simulans 转基因的三龄幼虫活动和 D. mauritiana 转基因的 5 天雄性活动低于任一亲本物种，