One hundred and fifty-seven lines isoallelic for esterase-6 were extracted from a natural population of D. melanogaster. The relative electrophoretic mobilities of Est6 allozymes in the different lines were determined by repeated pair-wise comparisons on high resolution cellulose acetate plates. Ten allozymic classes were resolved among the lines where only four, Est6-VF, Est6-F' Est6-F and Est6-S, had been evident using standard electrophoretic procedures. The additional variation resulted from the subdivision of Est6-F and Est6-S into three and five classes respectively. Genetic analyses confirmed that the additional classes mapped at or near the Est6 locus. Two of the additional classes occurring at low population frequencies were associated with significantly higher third chromosome viabilities. The most frequent class within Est6-F was in significant gametic disequilibrium with the linked polymorphic inversion In(3L)P. The same high resolution procedures were applied to 13 lines of D.melanogaster representing six Est6 thermostability variants. Different thermostability variants did not group consistently according to electrophoretic classes. This implies that the two criteria are detecting protein variation independently from each other and that much greater variation occurs than is detected by either criterion alone.

中文翻译：

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黑腹果蝇天然种群中酯酶 6 基因座的高分辨率电泳变异

从 D. melanogaster 的自然种群中提取了 157 个酯酶 6 等位基因的品系。Est6 同工酶在不同品系中的相对电泳迁移率通过在高分辨率醋酸纤维素板上重复成对比较来确定。在使用标准电泳程序时只有四种，即 Est6-VF、Est6-F'、Est6-F 和 Est6-S 的品系中分离出 10 个异酶类。额外的变化是由于将 Est6-F 和 Est6-S 分别细分为三个和五个类别。遗传分析证实，额外的类别位于或靠近 Est6 基因座。出现在低种群频率的另外两个类别与显着更高的第三染色体活力相关。Est6-F 中最常见的类别与关联的多态性反转 In(3L)P 处于显着的配子不平衡状态。相同的高分辨率程序应用于代表六种 Est6 热稳定性变体的 13 种黑腹果蝇。根据电泳分类，不同的热稳定性变体没有一致地分组。这意味着这两个标准正在相互独立地检测蛋白质变异，并且比单独使用任一标准检测到的变异要大得多。