The term Elasmobranchs or Chondrichthyans refer to the group of marine organisms include sharks, skates, rays and chimeras. Due to high demand most of the Elasmobranch species depleted drastically in recent years. Being the apex predators in the marine environment most of the Chondrichthyan species are vulnerable with some risk of extinction. Accurate identification of species is prerequisite for conservation and management of these declining resources. In this context, character-based identification methods are of immense use which classify specimen to species using classification rules that compactly characterized species in terms of key diagnostic nucleotides in the selected gene sequences. In the present study, a total of 82 species of elasmobranches distributed in Indian waters is taken as the target group. A total of 20–30 mitochondrial CO1 sequences of each species were downloaded and modified. Using the BLOG2.0 software, species specific diagnostic nucleotides were identified for the selected group of species. Based on identified diagnostic nucleotide sites, species-specific probes with a length range of 23–30 bp were designed. These probes could be useful in detecting species of interest in customized microarrays (DNA chip). The technique is a powerful tool to obtain a significant amount of accurate information quickly and conveniently and could be used for forensic applications and conservation of elasmobranches in Indian waters.

术语E或软骨鱼类是指包括鲨鱼，滑冰，rays和嵌合体在内的海洋生物。由于需求旺盛，近年来，大多数Elasmobranch物种都急剧减少。作为海洋环境中的顶级掠食者，大部分的Chondrichthyan物种很容易遭受灭绝的威胁。准确识别物种是保护和管理这些资源减少的前提。在这种情况下，基于字符的识别方法非常有用，该方法使用分类规则将标本分类为物种，该分类规则根据所选基因序列中的关键诊断核苷酸对物种进行了精确表征。在本研究中，将分布在印度水域的总共82种弹性支流作为目标群体。每个物种总共下载并修改了20–30个线粒体CO1序列。使用BLOG2.0软件，为选定的物种组识别了物种特异性诊断核苷酸。基于确定的诊断核苷酸位点，设计了长度范围为23–30 bp的物种特异性探针。这些探针可用于检测定制微阵列（DNA芯片）中的目标物种。该技术是一种功能强大的工具，可快速，方便地获取大量准确的信息，可用于法医应用和印度水域的弹性支流的保存。基于确定的诊断核苷酸位点，设计了长度范围为23–30 bp的物种特异性探针。这些探针可用于检测定制微阵列（DNA芯片）中的目标物种。该技术是一种功能强大的工具，可快速，方便地获取大量准确的信息，可用于法医应用和印度水域的弹性支流的保存。基于确定的诊断核苷酸位点，设计了长度范围为23–30 bp的物种特异性探针。这些探针可用于检测定制微阵列（DNA芯片）中的目标物种。该技术是一种功能强大的工具，可快速，方便地获取大量准确的信息，可用于法医应用和印度水域的弹性支流的保存。