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DNA barcoding of shrimps from a mangrove biodiversity hotspot.
Mitochondrial DNA Part A ( IF 1.1 ) Pub Date : 2019-04-23 , DOI: 10.1080/24701394.2019.1597073
Jamsari Amirul Firdaus Jamaluddin 1 , Noor Adelyna Mohammed Akib 1, 2 , Siti Zuliana Ahmad 1 , Siti Amalia Aisyah Abdul Halim 1 , Noor Khalidah Abdul Hamid 1 , Siti Azizah Mohd Nor 1, 3
Affiliation  

A total of 74 shrimp specimens were sequenced at a 584 bp segment of the cytochrome oxidase subunit I (COI) gene to examine patterns of DNA barcode variation in a mangrove biodiversity hotspot. The Maximum Likelihood tree, barcode gap analysis, Automatic Barcode Gap Discovery analysis and sequence comparisons with data available from Barcode of Life Data System and GenBank recovered 18 taxa of which 15 were identified to species level, 2 at genus level and a single taxon at order level. Two deep mitochondrial DNA lineage divergences were found in the giant tiger prawn, Penaeus monodon. It is suggested that one of the lineages is a consequence of an introduction from aquaculture activity. These results have provided a reliable barcode library for cataloguing shrimps in this area.



中文翻译:

红树林生物多样性热点中虾的DNA条形码。

总共74个虾标本在细胞色素氧化酶亚基I(COI)基因的584 bp片段上测序,以检查红树林生物多样性热点中DNA条码变异的模式。最大似然树,条形码间隙分析,自动条形码空位发现分析以及序列比较(可从生命条形码系统和GenBank获得的数据)回收了18个分类单元,其中15个被识别为物种级别,2个为属级别,单个分类为定序水平。在巨型对虾斑节对虾中发现了两个深层的线粒体DNA谱系差异。有人提出,其中一个血统是水产养殖活动引种的结果。这些结果为该区域的虾分类提供了可靠的条形码库。

更新日期:2019-04-23
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