ABSTRACT LINC complexes (Linker of Nucleoskeleton and Cytoskeleton), consisting of inner nuclear membrane SUN (Sad1, UNC-84) proteins and outer nuclear membrane KASH (Klarsicht, ANC-1, and Syne Homology) proteins, are essential for nuclear positioning, cell migration and chromosome dynamics. To test the in vivo functions of conserved interfaces revealed by crystal structures, Cain et al used a combination of Caenorhabditis elegans genetics, imaging in cultured NIH 3T3 fibroblasts, and Molecular Dynamic simulations, to study SUN-KASH interactions. Conserved aromatic residues at the -7 position of the C-termini of KASH proteins and conserved disulfide bonds in LINC complexes play important roles in force transmission across the nuclear envelope. Other properties of LINC complexes, such as the helices preceding the SUN domain, the longer coiled-coils spanning the perinuclear space and higher-order organization may also function to transmit mechanical forces generated by the cytoskeleton across the nuclear envelope.

中文翻译：

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SUN/KASH 相互作用促进跨核膜的力传递

摘要 LINC 复合物（核骨架和细胞骨架的接头），由内核膜 SUN（Sad1、UNC-84）蛋白和外核膜 KASH（Klarsicht、ANC-1 和 Syne Homology）蛋白组成，对于核定位、细胞迁移和染色体动力学。为了测试晶体结构揭示的保守界面的体内功能，Cain 等人结合使用秀丽隐杆线虫遗传学、培养的 NIH 3T3 成纤维细胞中的成像和分子动力学模拟来研究 SUN-KASH 相互作用。KASH 蛋白 C 末端 -7 位置的保守芳香残基和 LINC 复合物中的保守二硫键在跨核膜的力传递中起着重要作用。LINC 复合物的其他特性，例如 SUN 结构域之前的螺旋，