BACKGROUND The rumen microbiota is one of the most complex consortia of anaerobes, involving archaea, bacteria, protozoa, fungi and phages. They are very effective at utilizing plant polysaccharides, especially cellulose and hemicelluloses. The most important hemicellulose decomposers are clustered with the genus Butyrivibrio. As the related species differ in their range of hydrolytic activities and substrate preferences, Butyrivibrio fibrisolvens was selected as one of the most effective isolates and thus suitable for proteomic studies on substrate comparisons in the extracellular fraction. The B. fibrisolvens genome is the biggest in the butyrivibria cluster and is focused on "environmental information processing" and "carbohydrate metabolism". METHODS The study of the effect of carbon source on B. fibrisolvens 3071 was based on cultures grown on four substrates: xylose, glucose, xylan, xylan with 25% glucose. The enzymatic activities were studied by spectrophotometric and zymogram methods. Proteomic study was based on genomics, 2D electrophoresis and nLC/MS (Bruker Daltonics) analysis. RESULTS Extracellular β-endoxylanase as well as xylan β-xylosidase activities were induced with xylan. The presence of the xylan polymer induced hemicellulolytic enzymes and increased the protein fraction in the interval from 40 to 80 kDa. 2D electrophoresis with nLC/MS analysis of extracellular B. fibrisolvens 3071 proteins found 14 diverse proteins with significantly different expression on the tested substrates. CONCLUSION The comparison of four carbon sources resulted in the main significant changes in B. fibrisolvens proteome occurring outside the fibrolytic cluster of proteins. The affected proteins mainly belonged to the glycolysis and protein synthesis cluster.

中文翻译：

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在不同碳源上生长的溶纤丁酸弧菌的酶活性和蛋白质组学特征的比较。

背景技术瘤胃微生物群是最复杂的厌氧菌群之一，涉及古生菌、细菌、原生动物、真菌和噬菌体。它们在利用植物多糖，尤其是纤维素和半纤维素方面非常有效。最重要的半纤维素分解者与丁酸弧菌属聚集在一起。由于相关物种的水解活性范围和底物偏好不同，因此，Butyrivibrio fibrisolvens 被选为最有效的分离物之一，因此适用于细胞外部分底物比较的蛋白质组学研究。B. fibrisolvens 基因组是丁酸菌群中最大的基因组，专注于“环境信息处理”和“碳水化合物代谢”。方法研究碳源对B. fibrisolvens 3071 基于在四种底物上生长的培养物：木糖、葡萄糖、木聚糖、含 25% 葡萄糖的木聚糖。通过分光光度法和酶谱法研究酶活性。蛋白质组学研究基于基因组学、2D 电泳和 nLC/MS (Bruker Daltonics) 分析。结果木聚糖诱导细胞外β-内切木聚糖酶和木聚糖β-木糖苷酶活性。木聚糖聚合物的存在诱导了半纤维素分解酶并增加了40至80 kDa区间内的蛋白质部分。对胞外溶纤芽孢杆菌 3071 蛋白质进行 nLC/MS 分析的 2D 电泳发现 14 种不同的蛋白质在测试底物上具有显着不同的表达。结论 四种碳源的比较导致 B. fibrisolvens 蛋白质组发生在纤维溶解蛋白簇之外。受影响的蛋白质主要属于糖酵解和蛋白质合成簇。