BACKGROUND Oxysterols have been shown to play a role in plaque formation while ischemia modified albumin (IMA) is widely accepted as an acute marker for ischemia. The effort test is one of the methods used to identify the presence of coronary artery disease. Thus, there may be a relationship between effort test result and the levels of IMA, 7-ketocholesterol (7-KC) and cholestane-3β,5α,6β-triol (C-triol). METHODS Thirty patients who underwent effort test and 30 healthy subjects were included in the study. IMA levels were determined with the albumin-cobalt binding test, 7-KC and C-triol levels were determined with LC-MS/MS. Among the patients, two subgroups were identified according to the results of the effort test, group 1 consisted of patients with a positive effort test (n = 12), and group 2 consisted of patients who had a negative effort test (n = 18). RESULTS 7-KC levels of patients were significantly higher compared to healthy subjects (39.87 ± 2.13 ng/mL, 20.26 ± 1.35 ng/mL; p=0.001). In patients, post-test 7-KC levels were significantly lower than pre-test levels (post-test vs. pre-test: 37.73 ± 2.44 ng/mL vs. 41.07 ± 2.18 ng/mL; p<0.001). There was a significant difference in post-test 7-KC levels among all study groups (negative, positive and healthy: 37.73 ± 2.44 ng/mL, 39.87 ± 2.13 ng/mL, 20.26 ± 1.35 ng/mL, respectively). There was no significant difference in IMA levels. CONCLUSIONS Patients with positive effort test had significantly higher levels of 7-KC. Additionally, after the effort test, the 7-KC value was reduced. 7-KC is a biomarker of oxidative damage and its value or changes before and after the effort test may be used as a biomarker in the diagnosis and follow-up of coronary artery disease.

中文翻译：

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努力试验对缺血修饰白蛋白、7-酮胆固醇和Cholestan-3β、5α、6β-三醇水平的影响及其在冠状动脉疾病诊断中的作用。


背景技术已证明氧甾醇在斑​​块形成中发挥作用，而缺血修饰白蛋白(IMA)被广泛认为是缺血的急性标志物。努力测试是用于识别是否存在冠状动脉疾病的方法之一。因此，努力测试结果与IMA、7-酮胆固醇(7-KC)和胆甾烷-3β,5α,6β-三醇(C-triol)水平之间可能存在关系。方法 30 名接受努力测试的患者和 30 名健康受试者纳入研究。 IMA水平通过白蛋白-钴结合测试测定，7-KC和C-三醇水平通过LC-MS/MS测定。在患者中，根据努力测试的结果确定了两个亚组，第 1 组由努力测试呈阳性的患者 (n = 12) 组成，第 2 组由努力测试呈阴性的患者 (n = 18) 组成。 。结果 患者的 7-KC 水平显着高于健康受试者（39.87 ± 2.13 ng/mL，20.26 ± 1.35 ng/mL；p=0.001）。在患者中，测试后 7-KC 水平显着低于测试前水平（测试后与测试前：37.73 ± 2.44 ng/mL vs. 41.07 ± 2.18 ng/mL；p<0.001）。所有研究组之间测试后 7-KC 水平存在显着差异（阴性、阳性和健康组：分别为 37.73 ± 2.44 ng/mL、39.87 ± 2.13 ng/mL、20.26 ± 1.35 ng/mL）。 IMA水平没有显着差异。结论 努力测试呈阳性的患者 7-KC 水平显着较高。另外，经过努力测试后，7-KC值降低了。 7-KC是氧化损伤的生物标志物，其在努力试验前后的值或变化可作为冠心病诊断和随访的生物标志物。