ABSTRACT During meiosis, homologous chromosomes undergo a dramatic movement in order to correctly align. This is a critical meiotic event but the molecular properties of this ‘chromosomal dance’ still remainunclear. We identified DEB-1 – an orthologue of mammalian vinculin – as a new component of the mechanistic modules responsible for attaching the chromosomes to the nuclear envelope as apart of the LINC complex. In early meiotic nuclei of C. elegans, DEB-1 is localized to the nuclear periphery and alongside the synaptonemal complex of paired homologues. Upon DEB-1 depletion, chromosomes attached to SUN-1 foci remain highly motile until late pachytene. Although the initiation of homologue pairing started normally, irregularities in the formation of the synaptonemal complex occur, and these results in meiotic defects such as increased number of univalents at diakinesis and high embryonic lethality. Our data identify DEB-1 as a new player regulating chromosome dynamics and pairing during meiotic prophase I.

中文翻译：

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秀丽隐杆线虫减数分裂中同源染色体的配对需要 DEB-1——哺乳动物纽蛋白的直向同源物

摘要 在减数分裂过程中，同源染色体会发生剧烈运动以正确对齐。这是一个关键的减数分裂事件，但这种“染色体舞蹈”的分子特性仍然不清楚。我们将 DEB-1（哺乳动物纽蛋白的直向同源物）鉴定为负责将染色体连接到核膜作为 LINC 复合体一部分的机制模块的新组成部分。在秀丽隐杆线虫的早期减数分裂细胞核中，DEB-1 位于核外围，并与配对同源物的联会复合体相邻。DEB-1 耗尽后，附着在 SUN-1 病灶上的染色体在粗线期晚期保持高度运动。尽管同源配对的启动正常开始，但会出现联会复合体形成的不规则性，这些会导致减数分裂缺陷，例如单价分裂的数量增加和胚胎致死率高。我们的数据将 DEB-1 确定为在减数分裂前期 I 期间调节染色体动力学和配对的新参与者。