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Triauxic growth of an oleaginous red yeast Rhodosporidium toruloides on waste 'extract' for enhanced and concomitant lipid and β-carotene production.
Microbial Cell Factories ( IF 6.4 ) Pub Date : 2018-11-21 , DOI: 10.1186/s12934-018-1026-4
Gunjan Singh 1 , Sweta Sinha 1 , K K Bandyopadhyay 1 , Mark Lawrence 2 , Ram Prasad , Debarati Paul 1
Affiliation  

BACKGROUND Vegetable 'mandi' (road-side vegetable market) waste was converted to a suitable fermentation medium for cultivation of oleaginous yeast Rhodosporidium toruloides by steaming under pressure. This cultivation medium derived from waste was found to be a comparatively better source of nutrients than standard culture media because it provided more than one type of usable carbon source(s) to yeast. RESULTS HPLC results showed that the extract contained glucose, xylose and glycerol along with other carbon sources, allowing triauxic growth pattern with preferably usage of glucose, xylose and glycerol resulting in enhanced growth, lipid and carotenoid production. Presence of saturated and unsaturated fatty acid methyl esters (FAMEs) (C14-20) in the lipid profile showed that the lipid may be transesterified for biodiesel production. CONCLUSION Upscaling these experiments to fermenter scale for the production of lipids and biodiesel and other industrially useful products would lead to waste management along with the production of value added commodities. The technique is thus environment friendly and gives good return upon investment.

中文翻译:

废物中的油性红色酵母圆环假单胞菌三倍体在提取物中的生长,以增加和同时产生脂质和β-胡萝卜素。

背景技术将蔬菜“ mandi”(路边蔬菜市场)废物转化为合适的发酵培养基,以通过在压力下进行蒸煮来培养油性酵母圆环假单胞菌。发现这种源自废物的培养基是比标准培养基相对更好的营养来源,因为它为酵母提供了不止一种类型的可用碳源。结果HPLC结果表明,提取物含有葡萄糖,木糖和甘油以及其他碳源,允许三态生长模式,优选使用葡萄糖,木糖和甘油,从而提高了生长,脂质和类胡萝卜素的产生。脂质谱中存在饱和和不饱和脂肪酸甲酯(FAME)(C14-20)表明,脂质可能被酯交换以生产生物柴油。结论将这些实验扩大到发酵罐规模以生产脂质和生物柴油以及其他工业上有用的产品将导致废物管理以及增值商品的生产。因此,该技术对环境友好,并具有良好的投资回报率。
更新日期:2018-11-19
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