Can f 1 belongs to the lipocalin superfamily and is considered to be an animal allergen. The immune response induced by Can f 1 in mice was compared with that induced by ovalbumin (OVA), a typical food allergen. Female BALB/c and C57BL/6 mice (6 weeks of age) were subcutaneously injected with Can f 1 or OVA with or without aluminum hydroxide (Alum) three times with intervals of two weeks. Serum levels of total IgE or antigen-specific IgE and production of IL13 and IFNγ from splenocytes were analyzed. Immunization with Can f 1 or OVA increased serum levels of both total IgE and antigen-specific IgE significantly irrespective of Alum. These results indicate that Can f 1 and OVA were able to induce allergic sensitization in mice. Splenocyte production of IL13 in mice immunized with Can f 1 or OVA with and without Alum were significantly increased after stimulation with each antigen. However, IL13 levels in the mice immunized with Can f 1 with Alum were significantly lower than those immunized without Alum. Increases in IFNγ levels after stimulation with Can f 1 or OVA were not remarkable. No influence of genetic backgrounds of BALB/c and C57BL/6 mice was found. Although Can f 1 induced Th2 type immune responses as was also the case for immunization with OVA, an inhibitory effect of Alum on induction of IL13 was observed only in mice immunized with Can f 1. These results suggest that the immune mechanism for allergic sensitization with Can f 1 is different from that with OVA.

中文翻译：

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比较对动物过敏原Can f 1和食物过敏原卵清蛋白敏感的小鼠的免疫反应。

Can f 1属于lipocalin超家族，被认为是动物过敏原。将Can f 1诱导的小鼠免疫应答与卵白蛋白（OVA）（一种典型的食物过敏原）诱导的免疫应答进行了比较。给雌性BALB / c和C57BL / 6小鼠（6周龄）皮下注射Can f 1或OVA，加或不加氢氧化铝（明矾），间隔2周，共3次。分析了血清总IgE或抗原特异性IgE以及脾细胞中IL13和IFNγ的产生。不论是否使用明矾，Can f 1或OVA免疫均可显着提高血清总IgE和抗原特异性IgE的水平。这些结果表明Can f 1和OVA能够诱导小鼠过敏性致敏。每种抗原刺激后，用Can f 1或OVA免疫（有或没有明矾）免疫的小鼠，脾脏中IL13的脾细胞生成显着增加。但是，用明矾的Can f 1免疫的小鼠的IL13水平明显低于不使用明矾的免疫的小鼠。用Can f 1或OVA刺激后，IFNγ水平没有明显增加。没有发现BALB / c和C57BL / 6小鼠的遗传背景的影响。尽管Can f 1可以像用OVA免疫一样诱导Th2型免疫反应，但仅在Can f 1免疫的小鼠中观察到了Alum对IL13诱导的抑制作用。这些结果表明，用Fac 1进行过敏性致敏的免疫机制。 f 1与OVA可以不同吗？用明矾的Can f 1免疫的小鼠的IL13水平明显低于不使用明矾的免疫的小鼠。用Can f 1或OVA刺激后，IFNγ水平没有明显增加。没有发现BALB / c和C57BL / 6小鼠的遗传背景的影响。尽管Can f 1可以像用OVA免疫一样诱导Th2型免疫反应，但是仅在Can f 1免疫的小鼠中观察到了Alum对IL13诱导的抑制作用。这些结果表明，用Fac 1进行过敏性致敏的免疫机制。 f 1与OVA可以不同吗？用明矾的Can f 1免疫的小鼠的IL13水平明显低于不使用明矾的免疫的小鼠。用Can f 1或OVA刺激后，IFNγ水平没有明显增加。没有发现BALB / c和C57BL / 6小鼠的遗传背景的影响。尽管Can f 1可以像用OVA免疫一样诱导Th2型免疫反应，但是仅在Can f 1免疫的小鼠中观察到了Alum对IL13诱导的抑制作用。这些结果表明，用Fac 1进行过敏性致敏的免疫机制。 f 1是否与OVA不同。