INTRODUCTION Ninety four residents of Kowary city (Poland) have been investigated for environmental radon exposure that ranged from 0.24 WLM to 9.6 WLM (activity concentration range: 35-2700 Bq/m3). Kowary was chosen because of uranium mineralisation in its close vicinity. METHOD Whole population studied was divided into two groups: exposed to low radon activity concentrations resulting in the exposure of ≤0.55 WLM (value corresponding to the exposure to 100 Bq/m3 during whole year), and exposed to high radon activity concentration (>0.55 WLM). In the two groups two selected biomarkers in blood were assessed: the cytokinesis-block micronucleus assay (CBMN) on peripheral blood lymphocytes (PBL), and the levels of anti-p53 antibodies in serum measured because some data indicate increased expression of the antibodies in individuals after exposure to DNA damaging agents including radon. The potential confounding factors known to influence micronuclei (MN) frequency were also measured in serum: vitamin B12, folic acid, as well as total calcium. RESULTS In the present study no significant correlation was found between MN frequency in PBL and radon exposure. Among all persons investigated only 11 had detectable levels of the anti-p53 antibodies, whereas only 3 persons had positive result. Therefore, the group was too small to perform any meaningful statistical analysis and to conclude on any association. Cigarette smoking did not significantly influence the number of MN. There was a significant positive correlation observed between MN frequency and age, as well as higher MN frequency was detected in women. CONCLUSION The problem of the radon exposure is still unresolved and needs further studies on bigger human cohorts in order to search for more sensitive biomarkers.

中文翻译：

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室内ow浓度升高的科瓦里市（波兰）居民外周血淋巴细胞的微核频率和血清中抗p53自身抗体的水平。

引言已对波兰科瓦里市的94位居民的环境ra暴露进行了调查，其环境ra暴露范围为0.24 WLM至9.6 WLM（活动浓度范围：35-2700 Bq / m3）。选择科瓦里是因为其附近有铀矿化。方法将研究的整个人群分为两组：暴露于低ra活度浓度导致≤0.55 WLM（值对应于全年暴露于100 Bq / m3）和暴露于高ra活度浓度（> 0.55） WLM）。在两组中，评估了血液中两种选择的生物标志物：外周血淋巴细胞（PBL）的胞质阻滞微核试验（CBMN），以及测量血清中抗p53抗体的水平，因为一些数据表明，暴露于DNA破坏剂（包括agents）后，个体中抗体的表达增加。在血清中还测定了已知的影响微核（MN）频率的潜在混杂因素：维生素B12，叶酸以及总钙。结果在本研究中，在PBL中的MN频率与ra暴露之间没有发现显着相关性。在所有接受调查的人中，只有11人检测到抗p53抗体水平，而只有3人具有阳性结果。因此，该小组太小，无法进行任何有意义的统计分析并得出任何关联的结论。吸烟并没有显着影响MN的数量。MN频率与年龄之间存在显着的正相关，而且女性的MN频率较高。结论exposure暴露的问题仍未解决，需要对更大的人群进行进一步研究，以寻找更敏感的生物标志物。