Several studies linked between pesticides exposure and development of liver cancer, through several mechanisms inform of genotoxicity, cytotoxicity, tumor promotion, immunotoxicity and hormonal actions. This study aimed to estimate novel biomarkers for early prediction of liver malignancy due to occupational exposure to pesticides in two groups of workers with different socioeconomic standard (highly educated urban researchers and low educated rural pesticides sprayers). This study included 50 urban researchers and 50 rural pesticides sprayers occupationally exposed to pesticides. They were compared with 50 non-exposed urban researchers and 50 non-exposed rural subjects. Several tumor biomarkers were estimated; P53 protein, Alfa fetoprotein (AFP), and Alpha-L-fucosidase (AFU). Additionally, telomerase enzyme activity, Relative telomere length (RTL), and DNA damage using comet assay were measured. Furthermore, the glutathione-S-Transferase (GST) gene polymorphisms were identified for both exposed groups. Statistical analysis revealed elevated level of tumor biomarkers among exposed subjects relative to control groups in spite of being within the normal range. Increase in the DNA damage was detected, with shortening of telomere length and decrease in telomerase enzyme activity in pesticides-exposed subjects compared to their controls. Most of these changes were related to the levels of butyrylcholinesterase. Subjects with GSTT1 genotype were suggested to be more susceptible to hepatic carcinogenicity. Telomere relative length and comets assay together with GST genes polymorphisms could be used as early predictors for liver cancer susceptibility among pesticides exposed workers.

中文翻译：

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由于职业性接触农药而引起的肝致癌性的早期预测。

几项研究通过多种机制将农药暴露与肝癌的发展联系起来，从而揭示了遗传毒性，细胞毒性，肿瘤促进作用，免疫毒性和激素作用。这项研究旨在评估新的生物标志物，以早期预测因社会经济水平不同的两组工人（受过高等教育的城市研究人员和受过低学历的农村农药喷雾器）而因职业性接触农药而引起的肝恶性肿瘤。这项研究包括50名职业暴露于农药的城市研究人员和50名农村农药喷雾器。将他们与50位未暴露的城市研究人员和50位未暴露的农村受试者进行了比较。估计了几种肿瘤生物标志物；P53蛋白，阿尔法甲胎蛋白（AFP）和Alpha-L-岩藻糖苷酶（AFU）。此外，端粒酶活性 使用彗星测定法测量了相对端粒长度（RTL）和DNA损伤。此外，确定了两个暴露组的谷胱甘肽-S-转移酶（GST）基因多态性。统计分析表明，尽管处于正常范围内，但与对照组相比，暴露对象的肿瘤生物标志物水平升高。与对照相比，在暴露于农药的受试者中，检测到DNA损伤增加，端粒长度缩短，端粒酶活性降低。这些变化大多数与丁酰胆碱酯酶的水平有关。建议具有GSTT1基因型的受试者更易患肝癌。